Department of Chemistry, University of Colorado, Boulder, Colorado 80309.
Plant Physiol. 1978 Sep;62(3):418-22. doi: 10.1104/pp.62.3.418.
This paper describes the isolation and characterization of rhamnogalacturonan II, a hitherto unobserved component of the primary cell walls of dicotyledonous plants. Rhamnogalacturonan II constitutes 3 to 4% of the primary cell walls of suspension-cultured sycamore (Acer pseudoplatanus) cells. Rhamnogalacturonan II is a very complex polysaccharide yielding, upon hydrolysis, 10 different monosaccharides including the rarely observed sugars apiose, 2-O-methylxylose, and 2-O-methylfucose. In addition, rhamnogalacturonan II is characterized by the rarely observed glycosyl interconnections of 2-linked glucuronosyl, 3,4-linked fucosyl, and 3-linked rhamnosyl residues. These glycosyl linkages have never previously been detected in primary sycamore cell walls. Evidence is presented which suggests that polysaccharides similar to rhamnogalacturonan II are present in the primary cell walls of the three other dicotyledonous plants examined.
本文描述了鼠李半乳糖醛酸聚糖 II 的分离和特性，它是双子叶植物初生细胞壁中迄今尚未观察到的成分。鼠李半乳糖醛酸聚糖 II 占悬浮培养的悬铃木（Acer pseudoplatanus）细胞初生细胞壁的 3%至 4%。鼠李半乳糖醛酸聚糖 II 是一种非常复杂的多糖，水解后可产生 10 种不同的单糖，包括罕见的糖芹糖、2-O-甲基木糖和 2-O-甲基岩藻糖。此外，鼠李半乳糖醛酸聚糖 II 的特征还在于 2 连接的葡萄糖醛酸基、3,4 连接的岩藻糖基和 3 连接的鼠李糖基残基的罕见糖苷键连接。这些糖苷键连接以前从未在初生悬铃木细胞壁中检测到过。有证据表明，在本文所研究的其他三种双子叶植物的初生细胞壁中也存在类似于鼠李半乳糖醛酸聚糖 II 的多糖。
