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豌豆子叶中膜结合糖基转移酶:镁离子和锰离子的差异影响。

Membrane-associated Glycosyl Transferases in Cotyledons of Pisum sativum: Differential Effects of Magnesium and Manganese Ions.

机构信息

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.

出版信息

Plant Physiol. 1978 Nov;62(5):766-72. doi: 10.1104/pp.62.5.766.

Abstract

In crude particulate fractions isolated from pea (Pisum sativum) cotyledons, the transfer of radioactivity from GDP-[(14)C]mannose to glycolipid appears to be preferentially stimulated by Mn(2+) while the transfer to lipid-free residue is enhanced by Mg(2+). In contrast, the transfer of radioactivity from UDP-N-acetyl-[(14)C]glucosamine to glycolipid shows preferential stimulation by Mg(2+) while the transfer to lipid-free residue prefers Mn(2+). These results are accounted for by the differential stimulation by Mg(2+) and Mn(2+) of glycosyl transferases associated with subcellular membranes which were separated by isopycnic sucrose density centrifugation.

摘要

在从豌豆(Pisum sativum)子叶中分离出的粗颗粒部分中,GDP-[(14)C]甘露糖的放射性转移似乎优先被 Mn(2+)刺激,而向无脂残留物的转移则被 Mg(2+)增强。相比之下,UDP-N-乙酰-[(14)C]葡萄糖胺向糖脂的放射性转移优先被 Mg(2+)刺激,而向无脂残留物的转移则优先被 Mn(2+)刺激。这些结果是由于 Mg(2+)和 Mn(2+)对与亚细胞膜相关的糖基转移酶的差异刺激所导致的,这些酶是通过等密度蔗糖密度离心分离的。

相似文献

6
Glycosylation of pea cotyledon membranes.豌豆子叶膜的糖基化。
Plant Physiol. 1980 Apr;65(4):648-57. doi: 10.1104/pp.65.4.648.

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