Department of Agronomy and Range Science, University of California, Davis, California 95616.
Plant Physiol. 1978 Dec;62(6):846-50. doi: 10.1104/pp.62.6.846.
Uptake of sucrose and hexoses by cotton (Gossypium hirsutum L.) hypocotyl segments from free space was shown to be an active, carrier-mediated process. Separate carriers existed for hexoses and sucrose. Accumulated sugars appeared in both soluble and insoluble fractions of the tissue. At optimum temperature and pH, sucrose uptake rate versus concentration was fit by a rectangular hyperbola with V(max) of 14 micromoles per gram fresh weight per hour and K(m) of 8 mm. Sucrose was the principal sugar found in the free space in vivo, and invertase activity was essentially absent from that space except after aging.
棉(Gossypium hirsutum L.)下胚轴片段从游离空间摄取蔗糖和己糖被证明是一种主动的、载体介导的过程。己糖和蔗糖有单独的载体。积累的糖出现在组织的可溶性和不溶性部分。在最适温度和 pH 值下,蔗糖摄取速率与浓度的关系符合矩形双曲线,V(max)为每克鲜重每小时 14 微摩尔,K(m)为 8 毫米。蔗糖是体内游离空间中发现的主要糖,除老化后外,游离空间中几乎不存在转化酶活性。