日内变化在多核糖体含量和蛋白质合成辣椒叶片中的应用。
Within-day Changes in the Polyribosome Content and in Synthesis of Proteins in Leaves of Capsicum annuum L.
机构信息
Department of Physiology and Environmental Studies, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, LE12 5RD, United Kingdom.
出版信息
Plant Physiol. 1978 Dec;62(6):907-11. doi: 10.1104/pp.62.6.907.
Capsicum annuum cv. California Wonder was grown in controlled environment with a 12-hour photoperiod. The polyribosome content of leaves varied from 60 to 72% of total ribosomes with the highest level occurring in the middle of the photoperiod and the lowest in the middle of the dark period. The variation was accounted for by changes in the content of large polyribosomes (hexamers and larger). There was no indication of an immediate effect on polyribosome content of light-on or light-off.The synthesis of proteins at two times in the 24-hour cycle was compared using a dual isotope technique. Statistically significant results were obtained that suggested that protein(s) with molecular weights of 26,000 daltons were preferentially synthesized in the photoperiod compared to the dark period. No evidence was found for the differential synthesis of proteins within the photoperiod.
甜椒 cv. 加利福尼亚奇迹在 12 小时光周期的控制环境中生长。叶片的多核糖体含量占总核糖体的 60%到 72%，其中最高水平出现在光周期的中间，最低水平出现在暗期的中间。这种变化是由大核糖体（六聚体和更大的核糖体）含量的变化引起的。光的开启或关闭对多核糖体含量没有直接影响的迹象。使用双同位素技术比较了 24 小时周期中两个时间点的蛋白质合成。得到了具有统计学意义的结果，表明与暗期相比，26000 道尔顿分子量的蛋白质优先在光周期中合成。在光周期内没有发现蛋白质的差异合成的证据。
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