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小麦中的胸苷磷酸化：分析从 ATP 到胸苷的磷酸基团转移。

Thymidine phosphorylation in wheat: analysis of phosphate transfer from ATP to thymidine.

机构信息

Department of Botany, University of Tennessee, Knoxville, Tennessee 37916.

出版信息

Plant Physiol. 1978 Dec;62(6):930-2. doi: 10.1104/pp.62.6.930.

DOI:10.1104/pp.62.6.930
PMID:16660642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1092258/
Abstract

Extracts of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi) seedlings contain thymidine-phosphorylating activity with ATP, ADP, or AMP and nucleotide hydrolase activity (ATP --> --> AMP). The synthesis of [(32)P]dTMP exclusively from [alpha-(32)P]ATP with none detectable from [gamma-(32)P]ATP demonstrates the absence of thymidine kinase and the presence of nucleoside phosphotransferase as the only observable thymidine-phosphorylating enzyme.

摘要

小麦（Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi）幼苗提取物含有与 ATP、ADP 或 AMP 一起作用的胸苷磷酸化活性和核苷酸水解酶活性（ATP-->>-->AMP）。仅用 [alpha-(32)P]ATP 合成 [(32)P]dTMP，而用 [gamma-(32)P]ATP 检测不到，这表明不存在胸苷激酶，而存在核苷磷酸转移酶，是唯一可观察到的胸苷磷酸化酶。

相似文献

1
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Plant Physiol. 1978 Dec;62(6):930-2. doi: 10.1104/pp.62.6.930.
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本文引用的文献

1
Thymidine-phosphorylating Activity in gamma-Plantlets: Separation of Onset of Activity from Deoxyribonucleic Acid Synthesis.γ-幼苗中的胸苷磷酸化活性：活性起始与脱氧核糖核酸合成的分离
Plant Physiol. 1973 May;51(5):984-6. doi: 10.1104/pp.51.5.984.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.大肠杆菌的脱氧胸苷激酶。I. 酶的纯化及某些性质
J Biol Chem. 1964 Jan;239:269-74.
4
Ion-exchange thin-layer chromatography. XV. Preparation, properties and applications of paper-like PEI-cellulose sheets.离子交换薄层色谱法。十五。类纸状聚乙烯亚胺纤维素薄板的制备、性质及应用。
J Chromatogr. 1966 Apr;22(1):110-7. doi: 10.1016/s0021-9673(01)97076-1.
5
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
6
Modes of nucleoside phosphorylation in plants: studies on the apparent thymidine kinase and true uridine kinase of seedlings.植物中核苷磷酸化的模式：对幼苗中表观胸苷激酶和真尿苷激酶的研究
Biochim Biophys Acta. 1972 Aug 25;277(2):235-44.
7
A nucleotide phosphotransferase from Escherichia coli. Purification and properties.来自大肠杆菌的一种核苷酸磷酸转移酶。纯化及特性
Biochemistry. 1973 Jul 31;12(16):3005-12. doi: 10.1021/bi00740a009.
8
Chromatographic separation of pyridine and adenine nucleotides on thin layers of poly(ethyleneimine) cellulose.
J Chromatogr. 1977 Jan 11;130:145-50. doi: 10.1016/s0021-9673(00)89790-3.