小麦中的胸苷磷酸化：分析从 ATP 到胸苷的磷酸基团转移。
Thymidine phosphorylation in wheat: analysis of phosphate transfer from ATP to thymidine.
机构信息
Department of Botany, University of Tennessee, Knoxville, Tennessee 37916.
出版信息
Plant Physiol. 1978 Dec;62(6):930-2. doi: 10.1104/pp.62.6.930.
Abstract
Extracts of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi) seedlings contain thymidine-phosphorylating activity with ATP, ADP, or AMP and nucleotide hydrolase activity (ATP --> --> AMP). The synthesis of [(32)P]dTMP exclusively from [alpha-(32)P]ATP with none detectable from [gamma-(32)P]ATP demonstrates the absence of thymidine kinase and the presence of nucleoside phosphotransferase as the only observable thymidine-phosphorylating enzyme.
摘要
小麦（Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi）幼苗提取物含有与 ATP、ADP 或 AMP 一起作用的胸苷磷酸化活性和核苷酸水解酶活性（ATP-->>-->AMP）。仅用 [alpha-(32)P]ATP 合成 [(32)P]dTMP，而用 [gamma-(32)P]ATP 检测不到，这表明不存在胸苷激酶，而存在核苷磷酸转移酶，是唯一可观察到的胸苷磷酸化酶。
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本文引用的文献
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Biochim Biophys Acta. 1972 Aug 25;277(2):235-44.
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