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1
Thymidine phosphotransferase and nucleotide phosphohydrolase of the fern Asplenium nidus. General properties and inhibition by adenosine 3':5'-cyclic monophosphate.鸟巢蕨的胸苷磷酸转移酶和核苷酸磷酸水解酶。一般特性及3':5'-环磷酸腺苷的抑制作用
Biochem J. 1976 Jun 1;155(3):571-81. doi: 10.1042/bj1550571.
2
Kinetic mechanism and inhibition of human liver thymidine kinase.人肝胸苷激酶的动力学机制及抑制作用
Biochim Biophys Acta. 1981 Aug 13;660(2):238-42. doi: 10.1016/0005-2744(81)90165-0.
3
Profiles of pyrimidine biosynthesis, salvage and degradation in disks of potato (Solanum tuberosum L.) tubers.马铃薯（Solanum tuberosum L.）块茎圆盘嘧啶生物合成、补救和降解的概况。
Planta. 2002 Sep;215(5):821-8. doi: 10.1007/s00425-002-0806-5. Epub 2002 Jun 21.
4
Adenosine- and ADP-phosphorylating capacity of herpes simplex virus-induced thymidine kinase enzyme complex.单纯疱疹病毒诱导的胸苷激酶酶复合物的腺苷和ADP磷酸化能力。
Intervirology. 1983;19(2):77-84. doi: 10.1159/000149341.
5
Regulation of a plant 5'(3')-ribonucleotide phosphohydrolase by cyclic nucleotides and pyrimidine, purine, and cytokinin ribosides.环核苷酸、嘧啶、嘌呤和细胞分裂素核糖苷对植物5'(3')-核糖核苷酸磷酸水解酶的调控
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1299-303. doi: 10.1073/pnas.71.4.1299.
6
Alteration of nucleoside transport of Chinese hamster cells by dibutyryl adenosine 3':5'-cyclic monophosphate.二丁酰腺苷 3':5'-环磷酸酯对中国仓鼠细胞核苷转运的影响
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3542-6. doi: 10.1073/pnas.69.12.3542.
7
Inhibition of 5'-nucleotidase from Ehrlich ascites-tumour cells by nucleoside triphosphates.核苷三磷酸对艾氏腹水瘤细胞5'-核苷酸酶的抑制作用。
Biochem J. 1969 Jan;111(1):83-9. doi: 10.1042/bj1110083.
8
[Some properties of cytoplasmic thymidine kinase and nucleoside phosphotransferase from rat liver].[大鼠肝脏细胞质胸苷激酶和核苷磷酸转移酶的一些特性]
Biokhimiia. 1977 Sep;42(9):1668-73.
9
Adenylic acid: deoxythymidine 5'-phosphotransferase: evidence for the existence of a novel herpes simplex virus-induced enzyme.腺苷酸：脱氧胸苷5'-磷酸转移酶：一种新型单纯疱疹病毒诱导酶存在的证据。
J Gen Virol. 1981 Apr;53(Pt 2):247-55. doi: 10.1099/0022-1317-53-2-247.
10
Inhibition of mammalian protein kinase and phosphodiesterase activities by a cyclic AMP-like compound isolated from higher plants.从高等植物中分离出的一种环腺苷酸类似物对哺乳动物蛋白激酶和磷酸二酯酶活性的抑制作用。
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4301-5. doi: 10.1073/pnas.75.9.4301.

引用本文的文献

1
Characterization of thymidine kinase and phosphorylation of deoxyribonucleosides in Chlamydomonas reinhardti.莱茵衣藻中胸苷激酶的特性及脱氧核糖核苷的磷酸化作用
Mol Gen Genet. 1979 Nov;176(3):399-409. doi: 10.1007/BF00333104.

本文引用的文献

1
Studies on the presence of adenosine cyclic 3':5'-monophosphate in oat coleoptiles.燕麦胚芽鞘中3':5'-环磷酸腺苷存在情况的研究。
Plant Physiol. 1975 Feb;55(2):346-51. doi: 10.1104/pp.55.2.346.
2
Failure to Detect Cyclic 3', 5'-Adenosine Monophosphate in Healthy and Crown Gall Tumorous Tissues of Vicia faba.未能在蚕豆健康组织和冠瘿瘤组织中检测到环3',5'-单磷酸腺苷
Plant Physiol. 1974 Sep;54(3):372-3. doi: 10.1104/pp.54.3.372.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
THE PROBLEM OF HALTING ENZYME ACTION WHEN EXTRACTING PLANT TISSUES.提取植物组织时酶活性停止的问题。
Anal Biochem. 1964 Dec;9:431-42. doi: 10.1016/0003-2697(64)90204-0.
5
DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.大肠杆菌的脱氧胸苷激酶。I. 酶的纯化及某些性质
J Biol Chem. 1964 Jan;239:269-74.
6
Phosphatase reactions during tissue extractions.组织提取过程中的磷酸酶反应。
Biochim Biophys Acta. 1963 Jul 2;74:135-7. doi: 10.1016/0006-3002(63)91340-4.
7
Nucleotide interconversions. X. Deoxyribo- and ribonucleoside 5'-phosphate synthesis via a phosphotransferase reaction in chick embryo extracts.核苷酸的相互转化。X. 通过鸡胚提取物中的磷酸转移酶反应合成脱氧核糖核苷和核糖核苷5'-磷酸
Arch Biochem Biophys. 1963 May;101:342-9. doi: 10.1016/s0003-9861(63)80022-3.
8
Specificity and phosphotransferase activity of purified placental alkaline phosphatase.纯化胎盘碱性磷酸酶的特异性和磷酸转移酶活性。
Arch Biochem Biophys. 1967 Sep;121(3):619-24. doi: 10.1016/0003-9861(67)90046-x.
9
Purification and properties of a nucleoside phosphotransferase from carrot.胡萝卜中一种核苷磷酸转移酶的纯化及性质
J Biol Chem. 1967 Oct 25;242(20):4834-40.
10
Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.胸苷激酶：粗糙脉孢菌及其他一些微生物中不存在该酶的证据，以及这与脱氧核糖核酸特异性标记的相关性。
J Gen Microbiol. 1968 Dec;54(2):307-17. doi: 10.1099/00221287-54-2-307.

鸟巢蕨的胸苷磷酸转移酶和核苷酸磷酸水解酶。一般特性及3':5'-环磷酸腺苷的抑制作用

Thymidine phosphotransferase and nucleotide phosphohydrolase of the fern Asplenium nidus. General properties and inhibition by adenosine 3':5'-cyclic monophosphate.

作者信息

Grivell A R, Jackson J F

出版信息

Biochem J. 1976 Jun 1;155(3):571-81. doi: 10.1042/bj1550571.

DOI:10.1042/bj1550571
PMID:182131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172879/
Abstract
  1. Extracts of several plant species contained nucleoside-AMP phosphotransferase activity. The ratio of activity with thymidine to that with uridine as nucleoside substrate was essentially constant, both between species and throughout plant development. Evidence is presented that the total thymidine-AMP phosphotransferase activity of the leaves of Asplenium nidus (bird's-nest fern) and of Helianthus tuberosus (Jerusalem artichoke) increases during maturation. 2. Thymidine-AMP phosphotransferase was purified 22-fold from a very rich source of this activity, extracts of A. nidus. 3. A broad specificity towards both nucleoside and nucleoside 5'-monophosphate substrates is displayed by this preparation, and the evidence suggests that all could be due to a single enzyme. 4. Nucleosides that act as substrates will also inhibit phosphotransfer to other nucleosides, with Ki values close to the corresponding Km values found when utilized as substrates. 5. Ca2+-activated ATP phosphohydrolase was separated from the phosphotransferase by differential complexing to Blue Dextran in the presence of urea, whereas an AMP phosphohydrolase activity was closely associated with thymidine-AMP phosphotransferase through all separation techniques used. 6. Metal ions did not activate either of the latter two activities, and 1,10-phenanthroline was found to inhibit the phosphotransferase. 7. Km values for AMP for the respective activities were 0.11 mM (thymidine phosphotransferase) and 0.20 mM (AMP phosphohydrolase) and for thymidine (phosphotransferase only) 0.88 mM. 8. 3':5'-Cyclic AMP was found to inhibit both phosphotransferase and AMP phosphohydrolase activities, with Ki values of 0.056 mM and 0.15 mM respectively. It is suggested that this inhibitor would be of value in revealing the existence of thymidine kinase in plant extracts with high thymidine phosphotransferase activity.
摘要
  1. 几种植物物种的提取物含有核苷 - AMP磷酸转移酶活性。以胸苷为核苷底物的活性与以尿苷为核苷底物的活性之比在不同物种之间以及整个植物发育过程中基本恒定。有证据表明，鸟巢蕨和菊芋叶片中的总胸苷 - AMP磷酸转移酶活性在成熟过程中会增加。2. 从富含该活性的来源——鸟巢蕨提取物中，将胸苷 - AMP磷酸转移酶纯化了22倍。3. 该制剂对核苷和核苷5'-单磷酸底物都具有广泛的特异性，证据表明这一切可能都归因于一种单一的酶。4. 作为底物的核苷也会抑制向其他核苷的磷酸转移，其抑制常数（Ki）值接近用作底物时发现的相应米氏常数（Km）值。5. 通过在尿素存在下与蓝色葡聚糖进行差异络合，将Ca2+激活的ATP磷酸水解酶与磷酸转移酶分离，而在所有使用的分离技术中，AMP磷酸水解酶活性都与胸苷 - AMP磷酸转移酶紧密相关。6. 金属离子不会激活后两种活性中的任何一种，并且发现1,10 - 菲咯啉会抑制磷酸转移酶。7. 各自活性的AMP的米氏常数（Km）值分别为0.11 mM（胸苷磷酸转移酶）和0.20 mM（AMP磷酸水解酶），而胸苷（仅针对磷酸转移酶）的米氏常数为0.88 mM。8. 发现3':5'-环AMP会抑制磷酸转移酶和AMP磷酸水解酶活性及其抑制常数（Ki）值分别为0.056 mM和0.15 mM。有人认为这种抑制剂在揭示具有高胸苷磷酸转移酶活性的植物提取物中胸苷激酶的存在方面具有价值。