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硅藻中肋骨条藻对硝酸盐的利用：II. 硝酸盐吸收的调节

Nitrate Utilization by the Diatom Skeletonema costatum: II. Regulation of Nitrate Uptake.

作者信息

Serra J L, Llama M J, Cadenas E

机构信息

Departamento de Bioquímica, Facultad de Ciencias, Universidad de Bilbao, Bilbao, Spain.

出版信息

Plant Physiol. 1978 Dec;62(6):991-4. doi: 10.1104/pp.62.6.991.

DOI:10.1104/pp.62.6.991
PMID:16660653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1092269/
Abstract

Nitrate utilization has been characterized in nitrogen-deficient cells of the marine diatom Skeletonema costatum. In order to separate nitrate uptake from nitrate reduction, nitrate reductase activity was suppressed with tungstate. Neither nitrite nor the presence of amino acids in the external medium or darkness affects nitrate uptake kinetics. Ammonium strongly inhibits carrier-mediated nitrate uptake, without affecting diffusion transfer. A model is proposed for the uptake and assimilation of nitrate in S. costatum and their regulation by ammonium ions.

摘要

已对海洋硅藻中肋骨条藻缺氮细胞的硝酸盐利用情况进行了表征。为了将硝酸盐吸收与硝酸盐还原区分开来，用钨酸盐抑制了硝酸还原酶活性。外部培养基中的亚硝酸盐、氨基酸的存在或黑暗均不影响硝酸盐吸收动力学。铵强烈抑制载体介导的硝酸盐吸收，但不影响扩散转运。提出了一个关于中肋骨条藻中硝酸盐吸收与同化及其受铵离子调控的模型。

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本文引用的文献

1
Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall.环境或发育变化会导致高等植物的许多酶活性上升或下降。
Science. 1969 Jul 25;165(3891):358-67. doi: 10.1126/science.165.3891.358.
2
Nitrate Utilization by the Diatom Skeletonema costatum: I. Kinetics of Nitrate Uptake.甲藻 Skeletonema costatum 对硝酸盐的利用：I. 硝酸盐摄取的动力学。
Plant Physiol. 1978 Dec;62(6):987-90. doi: 10.1104/pp.62.6.987.
3
Regulation of Nitrate Uptake in Penicillium chrysogenum by Ammonium Ion.铵离子对产黄青霉硝酸盐摄取的调控。
Plant Physiol. 1973 Oct;52(4):362-7. doi: 10.1104/pp.52.4.362.
4
The effect of tungstate on nitrate assimilation in higher plant tissues.钨酸盐对高等植物组织中硝酸盐同化作用的影响。
Plant Physiol. 1969 Aug;44(8):1197-9. doi: 10.1104/pp.44.8.1197.
5
Regulation of the nitrate assimilation pathway in cultured tobacco cells. 3. The nitrate uptake system.培养烟草细胞中硝酸盐同化途径的调控。3. 硝酸盐吸收系统。
Biochim Biophys Acta. 1971 Feb 23;230(2):362-72. doi: 10.1016/0304-4165(71)90223-6.
6
Nitrate transport system in Neurospora crassa.粗糙脉孢菌中的硝酸盐转运系统。
J Bacteriol. 1974 Apr;118(1):259-69. doi: 10.1128/jb.118.1.259-269.1974.
7
Characterization of a membrane-bound nitrate reductase from Azotobacter chroococcum.来自褐球固氮菌的一种膜结合硝酸还原酶的特性分析。
Biochem Biophys Res Commun. 1977 Apr 11;75(3):682-8. doi: 10.1016/0006-291x(77)91526-1.
8
Ferredoxin-dependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans.费氏铁氧还蛋白依赖的集胞藻颗粒对硝酸盐和亚硝酸盐的光合还原作用。
Mol Cell Biochem. 1976 Feb 25;10(3):161-9. doi: 10.1007/BF01731687.