从 Fremyella diplosiphon 中纯化和表征 RNA 聚合酶。
Purification and Characterization of RNA Polymerase from Fremyella diplosiphon.
机构信息
Harvard University, The Biological Laboratories, 16 Divinity Avenue, Cambridge, Massachusetts 02138.
出版信息
Plant Physiol. 1978 Dec;62(6):995-9. doi: 10.1104/pp.62.6.995.
Abstract
We have purified and characterized a DNA-dependent RNA polymerase from the blue-green alga Fremyella diplosiphon. This enzyme, purified by gel filtration, DEAE-cellulose chromatography, and glycerol gradient centrifugation, is comprised of five polypeptide subunits. Their masses are 161,000, 134,000, 91,000, 72,000, and 41,000 daltons. Preparative electrophoresis of the purified enzyme on nondenaturing gels separates the 41,000-dalton polypeptide from the rest of the enzyme. The enzyme is extremely labile in the presence of a variety of salts of strong acids and bases; the purification procedure was devised to avoid exposure to such compounds.
摘要
我们从蓝绿藻鱼腥藻中纯化并鉴定了一种依赖于 DNA 的 RNA 聚合酶。该酶经凝胶过滤、DEAE-纤维素层析和甘油梯度离心纯化，由五个多肽亚基组成。它们的分子量分别为 161000、134000、91000、72000 和 41000 道尔顿。在非变性凝胶上对纯化酶进行制备电泳，将 41000 道尔顿的多肽与酶的其余部分分离开来。该酶在存在各种强酸和强碱盐的情况下极不稳定；纯化程序的设计是为了避免接触这些化合物。
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