玉米的RNA聚合酶：核RNA聚合酶
RNA polymerases of maize: nuclear RNA polymerases.
作者信息
Strain G C, Mullinix K P, Bogorad L
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2647-51. doi: 10.1073/pnas.68.11.2647.
Abstract
Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays. The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH(4))(2)SO(4), respectively. Both enzymes prefer maize nuclear DNA as a template; they are also more active in the presence of Mg(++) than Mn(++) and are inhibited by (NH(4))(2)-SO(4) or KCl. Neither enzyme is inhibited by rifamycin SV. Enzyme II is strongly inhibited by alpha-amanitin, whereas enzyme I is not significantly affected. Their ability to use native and denatured DNA as templates varies according to the extent and method of purification of the polymerase. Furthermore, enzyme II can be resolved by DEAE-chromatography or glycerol-gradient centrifugation into two components, one of which prefers native DNA, while the other prefers denatured DNA.
摘要
已从玉米叶片中纯化出两种核源的依赖DNA的RNA聚合酶。这两种酶可在DEAE - 纤维素柱上分离。酶I和酶II分别用0.08和0.20 M硫酸铵洗脱。两种酶都更倾向于以玉米核DNA作为模板；它们在镁离子存在下比在锰离子存在下更具活性，并且会受到硫酸铵或氯化钾的抑制。两种酶均不受利福霉素SV的抑制。酶II受到α - 鹅膏蕈碱的强烈抑制，而酶I则未受到显著影响。它们使用天然DNA和变性DNA作为模板的能力会根据聚合酶的纯化程度和方法而有所不同。此外，酶II可通过DEAE - 色谱法或甘油梯度离心法解析为两个组分，其中一个组分更倾向于天然DNA，而另一个则更倾向于变性DNA。
相似文献
1
RNA polymerases of maize: nuclear RNA polymerases.玉米的RNA聚合酶：核RNA聚合酶
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2647-51. doi: 10.1073/pnas.68.11.2647.
2
RNA polymerases of maize: partial purification and properties of the chloroplast enzyme.玉米的RNA聚合酶：叶绿体酶的部分纯化及特性
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2412-6. doi: 10.1073/pnas.68.10.2412.
3
Transcription in yeast: separation and properties of multiple FNA polymerases.酵母中的转录：多种FNA聚合酶的分离与特性
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1702-6. doi: 10.1073/pnas.69.7.1702.
4
Isolation and purification of RNA polymerases from rye embryos.从黑麦胚胎中分离和纯化RNA聚合酶。
Biochim Biophys Acta. 1975 Apr 16;390(1):105-16. doi: 10.1016/0005-2787(75)90013-1.
5
Purification and properties of two RNA polymerases from Physarum polycephalum.多头绒泡菌两种RNA聚合酶的纯化及性质
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1174-7. doi: 10.1073/pnas.71.4.1174.
6
RNA polymerases of maize. Purification and molecular structure of DNA-dependent RNA polymerase II.玉米的RNA聚合酶。依赖DNA的RNA聚合酶II的纯化及分子结构
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2386-90. doi: 10.1073/pnas.70.8.2386.
7
Purification and characterization of RNA polymerase I from a higher plant.高等植物RNA聚合酶I的纯化与特性分析
Biochim Biophys Acta. 1976 Feb 5;418(3):344-57. doi: 10.1016/0005-2787(76)90296-3.
8
A facile procedure for purifying maize chloroplast RNA polymerase from whole cell homogenates.一种从全细胞匀浆中纯化玉米叶绿体RNA聚合酶的简便方法。
Biochim Biophys Acta. 1980 Aug 26;609(1):14-30. doi: 10.1016/0005-2787(80)90197-5.
9
Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores.与匍枝根霉孢子萌发相关的可溶性核糖核酸聚合酶的变化。
Biochim Biophys Acta. 1972 Jun 22;272(1):44-52. doi: 10.1016/0005-2787(72)90031-7.
10
Comparison among DNA polymerases 1, 2 and 3 from maize embryo axes. A DNA primase activity copurifies with DNA polymerase 2.玉米胚轴中DNA聚合酶1、2和3之间的比较。一种DNA引发酶活性与DNA聚合酶2共纯化。
Plant Mol Biol. 1997 Feb;33(3):445-55. doi: 10.1023/a:1005753711459.
引用本文的文献
1
Transcribed enhancer sequences are required for maize p1 paramutation.转录增强子序列是玉米 p1 顺式突变所必需的。
Genetics. 2024 Jan 3;226(1). doi: 10.1093/genetics/iyad178.
2
RNA-mediated trans-communication can establish paramutation at the b1 locus in maize.RNA 介导的跨交流可以在玉米的 b1 基因座上建立拟突变。
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):12986-91. doi: 10.1073/pnas.1007972107. Epub 2010 Jun 29.
3
Chilling-Induced Ethylene Production in Cucumbers (Cucumis sativus L.).低温胁迫下黄瓜（Cucumis sativus L.）乙烯的产生。
Plant Physiol. 1982 Feb;69(2):424-7. doi: 10.1104/pp.69.2.424.
4
Comparison of Large Subunits of Type II DNA-dependent RNA Polymerases from Higher Plants.高等植物II型DNA依赖性RNA聚合酶大亚基的比较
Plant Physiol. 1979 Oct;64(4):671-3. doi: 10.1104/pp.64.4.671.
5
Purification and Characterization of RNA Polymerase from Fremyella diplosiphon.从 Fremyella diplosiphon 中纯化和表征 RNA 聚合酶。
Plant Physiol. 1978 Dec;62(6):995-9. doi: 10.1104/pp.62.6.995.
6
Sequence of Reactivation of Ribonucleic Acid Synthesis during Early Germination of the Maize Embryo.玉米胚胎早期萌发过程中 RNA 合成的再激活顺序。
Plant Physiol. 1976 Apr;57(4):632-9. doi: 10.1104/pp.57.4.632.
7
Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.从大豆下胚轴中分离核仁及定位 RNA 聚合酶 I。
Plant Physiol. 1975 Dec;56(6):850-2. doi: 10.1104/pp.56.6.850.
8
Ethylene-forming Systems in Etiolated Pea Seedling and Apple Tissue.黑暗中豌豆幼苗和苹果组织中的乙烯形成系统。
Plant Physiol. 1975 Jun;55(6):1074-8. doi: 10.1104/pp.55.6.1074.
9
Separation and partial characterization of two ribonucleic Acid polymerases from pea seedlings.豌豆幼苗中两种核糖核酸聚合酶的分离与部分特性鉴定
Plant Physiol. 1974 Sep;54(3):356-9. doi: 10.1104/pp.54.3.356.
10
Separation and partial characterization of multiple ribonucleic Acid polymerases from soybean hypocotyl.大豆下胚轴中多种核糖核酸聚合酶的分离与部分特性分析。
Plant Physiol. 1974 Sep;54(3):349-55. doi: 10.1104/pp.54.3.349.
本文引用的文献
1
Histone, a suppressor of chromosomal RNA synthesis.组蛋白，一种染色体RNA合成的抑制剂。
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1216-22. doi: 10.1073/pnas.48.7.1216.
2
Studies on the pathogenesis of liver necrosis by alpha-amanitin. Effect of alpha-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nuclei.α-鹅膏蕈碱致肝坏死的发病机制研究。α-鹅膏蕈碱对小鼠肝细胞核核糖核酸合成及核糖核酸聚合酶的影响。
Biochem J. 1967 Nov;105(2):779-82. doi: 10.1042/bj1050779.
3
Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids.核糖核酸酶H。一种降解DNA-RNA杂交体中RNA部分的酶。
Eur J Biochem. 1970 Jun;14(2):278-83. doi: 10.1111/j.1432-1033.1970.tb00287.x.
4
A factor from calf thymus stimulating DNA-dependent RNA polymerase isolated from this tissue.一种来自小牛胸腺的因子，可刺激从该组织中分离出的依赖DNA的RNA聚合酶。
Eur J Biochem. 1970 Jun;14(2):270-7. doi: 10.1111/j.1432-1033.1970.tb00286.x.
5
Factors and rifampicin influencing RNA polymerase isolated from chromatin of eukaryotic cell.影响从真核细胞染色质中分离出的RNA聚合酶的因素及利福平
Biochem Biophys Res Commun. 1970 Sep 10;40(5):1194-200. doi: 10.1016/0006-291x(70)90922-8.
6
Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.真核生物中DNA依赖性RNA聚合酶的多种形式。
Nature. 1969 Oct 18;224(5216):234-7. doi: 10.1038/224234a0.
7
Specific inhibitors of the three RNA polymerases from the aquatic fungus Blastocladiella emersonii.水生真菌艾美球囊霉三种RNA聚合酶的特异性抑制剂。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):338-41. doi: 10.1073/pnas.68.2.338.
8
Specific nucleolar and nucleoplasmic RNA polymerases.特定的核仁与核质RNA聚合酶。
Proc Natl Acad Sci U S A. 1970 Mar;65(3):675-82. doi: 10.1073/pnas.65.3.675.
9
RNA polymerases of maize: partial purification and properties of the chloroplast enzyme.玉米的RNA聚合酶：叶绿体酶的部分纯化及特性
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2412-6. doi: 10.1073/pnas.68.10.2412.
10
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.一种大规模纯化大肠杆菌脱氧核糖核酸依赖性核糖核酸聚合酶的新方法。
J Biol Chem. 1969 Nov 25;244(22):6160-7.
Zotero 插件