小麦胚早期萌发过程中UDP-葡萄糖的快速增加。
Rapid Increase in UDP-Glucose during Early Wheat Embryo Germination.
作者信息
Cheung C P, Suhadolnik R J
机构信息
Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
出版信息
Plant Physiol. 1979 Jan;63(1):146-8. doi: 10.1104/pp.63.1.146.
Abstract
The cellular content of UDP-glucose in isolated wheat (Triticum aestivum L.) embryo increases 8-fold during the first 40 minutes of imbibition. An additional 3-fold increase in the amount of UDP-glucose was observed in the next 5 hours of germination. This communication also describes a unique, quantitative method to achieve a high sensitivity in a direct determination of UDP-glucose with Na [(32)P]pyrophosphate and UDP-glucose pyrophosphorylase. The sensitivity of the assay for UDP-glucose is 10 picomoles.
摘要
在离体小麦（普通小麦）胚中，UDP - 葡萄糖的细胞含量在吸水的最初40分钟内增加了8倍。在随后的5小时萌发过程中，UDP - 葡萄糖的量又增加了3倍。本通讯还描述了一种独特的定量方法，该方法利用Na[(32)P]焦磷酸和UDP - 葡萄糖焦磷酸化酶直接测定UDP - 葡萄糖时具有高灵敏度。UDP - 葡萄糖测定法的灵敏度为10皮摩尔。
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