United States Department of Agriculture, Science and Education Administration, Department of Botany, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1979 Feb;63(2):237-43. doi: 10.1104/pp.63.2.237.
we have compared chloroplast lamellae isolated from a chlorophyll-b-less mutant and wild type barley (Hordeum vulgare). The results demonstrate that: (a) one of the two major polypeptides comprising the lightharvesting complex (LHC) is present in the chlorophyll-b-less mutant; (b) higher cation concentrations are required to maintain grana stacks in the mutant; and (c) cation effects on excitation energy distribution are present in the chlorophyll-b-less mutant but are reduced in amount and are dependent on higher concentrations of cations.We interpret these data to support the concept that the LHC mediates cation-induced grana stacking and cation regulation of excitation energy distribution between photosystems I and Ii in chloroplast lamellae. A partial LHC complement in the mutant alters the quantitative cation requirement for both phenomena, but not the over-all qualitative response.
我们比较了从叶绿素 b 缺失突变体和野生型大麦（Hordeum vulgare）中分离的叶绿体片层。结果表明：（a）组成光捕获复合物（LHC）的两个主要多肽之一存在于叶绿素 b 缺失突变体中；（b）需要更高的阳离子浓度来维持突变体中的粒层堆叠；（c）阳离子对激发能分布的影响存在于叶绿素 b 缺失突变体中，但数量减少，并且依赖于更高的阳离子浓度。我们解释这些数据支持这样的概念，即 LHC 介导阳离子诱导的粒层堆叠和阳离子对叶绿体片层中光系统 I 和 II 之间激发能分布的调节。突变体中 LHC 的部分补充改变了这两种现象的阳离子需求的定量，但不改变整体定性反应。
