Carnegie Institution of Washington, 94305, Stanford, CA, USA.
Photosynth Res. 1982 Jan;3(1):19-30. doi: 10.1007/BF00030046.
The absorption (640-710 nm) and fluorescence emission (670-710 nm) spectra (77 K) of wild-type and Chl b-less, mutant, barley chloroplasts grown under either day or intermittent light were analysed by a RESOL curve-fitting program. The usual four major forms of Chl a at 662, 670, 678 and 684 nm were evident in all of the absorption spectra and three major components at 686, 693 and 704 nm in the emission spectra. A broad Chl a component band at 651 nm most likely exists in all chlorophyll spectra in vivo. The results show that the mutant lacks not only Chl b, but also the Chl a molecules which are bound to the light-harvesting, Chl a/b, protein complex of normal plants. It also appears that the absorption spectrum of this antenna complex is not modified appreciably by its isolation from thylakoid membranes.
野生型和 Chl b 缺失突变体大麦叶绿体在持续光照或间歇光照条件下生长，其吸收光谱（640-710nm）和荧光发射光谱（670-710nm）（77K）用 RESOL 曲线拟合程序进行了分析。在所有吸收光谱中都可以明显看出通常的四种主要的 Chl a 形式：662nm、670nm、678nm 和 684nm，在发射光谱中有三个主要成分：686nm、693nm 和 704nm。在所有活体叶绿素光谱中很可能存在一个宽的 Chl a 组件带在 651nm 处。结果表明，突变体不仅缺乏 Chl b，而且还缺乏与正常植物的光捕获 Chl a/b 蛋白复合物结合的 Chl a 分子。看来，这个天线复合物的吸收光谱在从类囊体膜中分离出来后并没有明显改变。
