Department of Horticulture, The Hebrew University of Jerusalem, Rehovot 76-100, Israel.
Plant Physiol. 1979 Feb;63(2):280-2. doi: 10.1104/pp.63.2.280.
The induction of callus formation in cultured buds of Shamouti orange (Citrus sinensis [L.] Osbeck) by abscisic acid (ABA) is a multiphasic process. (Altman, and Goren 1974 Physiol Plant 32: 55.) A study of the mediation by ethylene on this effect of ABA was undertaken. It was found that: (a) ethylene and (2-chloroethyl) phosphonic acid, as well as ABA, induced callus formation; (b) callus induction is best attained when explants are exposed to ethylene during the 1st day after excision; and (c) ABA-induced callus formation is inhibited by rhizobitoxine analog, an inhibitor of ethylene biosynthesis. It is concluded that the effect of ABA on callus formation is mediated via ethylene.
ABA 诱导温州蜜柑（Citrus sinensis [L.] Osbeck）培养芽的愈伤组织形成是一个多相过程。（Altman 和 Goren，1974 年，《植物生理学》32:55），本研究探讨了乙烯对 ABA 这种效应的介导作用。结果发现：（a）乙烯和 2-氯乙基膦酸以及 ABA 都能诱导愈伤组织形成；（b）当外植体在切除后第 1 天暴露于乙烯时，愈伤组织诱导效果最佳；（c）ABA 诱导的愈伤组织形成被乙烯生物合成抑制剂——根瘤菌素类似物所抑制。结论是，ABA 对愈伤组织形成的影响是通过乙烯介导的。
