Tompkins M B, Nelson P D, English R V, Novotney C
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
J Am Vet Med Assoc. 1991 Nov 15;199(10):1311-5.
Feline leukemia virus and feline immunodeficiency virus (FIV) are lymphotropic retroviruses that cause a wide range of diseases in domestic cats. Although it is known that both viruses are capable of infecting T lymphocytes and that infected cats are lymphopenic, it was not known how infection with either virus might alter specific lymphocyte subpopulations. Using a panel of monoclonal antibodies to feline lymphocyte subpopulations, we examined, by use of flow cytometric analysis, lymphocyte changes in cats naturally infected with FeLV or FIV and explored the early stages in the immunopathogenesis of experimentally induced infection with these viruses. Both groups of naturally infected cats had T-cell lymphopenia. In the FIV-infected cats, the T-cell decrease was principally attributable to loss of CD4+ cells, whereas CD8+ and B-cell numbers remained normal. This led to inversion of the CD4+ to CD8+ ratio in these cats. In contrast, the T-cell lymphopenia in FeLV-infected cats resulted from decrease in CD4+ and CD8+ cells, which led to a CD4+ to CD8+ ratio within normal limits. Experimentally induced infection with these 2 viruses supported these findings. Infection with FIV induced early (10 weeks after infection), chronic inversion of the CD4+ to CD8+ ratio. In contrast, infection with FeLV did not alter CD4+ to CD8+ ratio in the first 20 weeks after infection.
猫白血病病毒和猫免疫缺陷病毒（FIV）是嗜淋巴细胞性逆转录病毒，可在家猫中引发多种疾病。虽然已知这两种病毒都能够感染T淋巴细胞，且受感染的猫会出现淋巴细胞减少，但此前并不清楚感染其中任何一种病毒会如何改变特定的淋巴细胞亚群。我们使用一组针对猫淋巴细胞亚群的单克隆抗体，通过流式细胞术分析，检测了自然感染猫白血病病毒（FeLV）或猫免疫缺陷病毒（FIV）的猫的淋巴细胞变化，并探究了实验性诱导感染这些病毒的免疫发病机制的早期阶段。两组自然感染的猫均出现T细胞淋巴细胞减少。在感染FIV的猫中，T细胞减少主要归因于CD4+细胞的丢失，而CD8+细胞和B细胞数量保持正常。这导致这些猫的CD4+与CD8+比值倒置。相比之下，感染FeLV的猫的T细胞淋巴细胞减少是由CD4+和CD8+细胞减少所致，这使得CD4+与CD8+比值处于正常范围内。对这两种病毒进行实验性诱导感染的结果支持了这些发现。感染FIV会导致早期（感染后10周）慢性的CD4+与CD8+比值倒置。相比之下，感染FeLV在感染后的前20周内并未改变CD4+与CD8+比值。
