Rogers C A
Department of Biology, Houston Baptist University, Houston, Texas 77074.
Plant Physiol. 1979 Feb;63(2):388-91. doi: 10.1104/pp.63.2.388.
Epidermal strips of Vicia faba were floated on 10 millimolar KCl at various temperatures and for several time periods. The diameter of the stomatal aperture was determined microscopically and K(+) content was estimated and expressed as the per cent of the guard cell stained. Stomatal opening was associated with increased K(+) in guard cells, but the quantitative association was modified both by time and temperature. At low temperatures (0-20 C) there was a prolonged Spannungsphase while at higher temperatures (30-45 C) motorphase was exhibited. During the motorphase there was a rapid opening of the stomates which was highly correlated with K(+) influx. At treatment periods of 360 minutes and temperatures higher than 25 C there appeared to be a maintenance phase during which K(+) concentration of the guard cells decreased without an equivalent decrease in aperture.
将蚕豆的表皮条漂浮在不同温度下的10毫摩尔氯化钾溶液中，并持续不同时间段。通过显微镜测定气孔孔径的直径，并估算钾离子含量，以保卫细胞染色的百分比表示。气孔开放与保卫细胞中钾离子增加有关，但这种定量关系会因时间和温度而改变。在低温（0-20℃）下，存在较长的紧张期，而在较高温度（30-45℃）下，则表现出运动期。在运动期，气孔迅速开放，这与钾离子内流高度相关。在处理360分钟且温度高于25℃时，似乎存在一个维持期，在此期间保卫细胞中的钾离子浓度下降，但孔径没有相应减小。
