蚕豆叶片半乳糖脂的合成：IV. 脂肪酸掺入主要甘油脂质的部位。
Galactolipid Synthesis in Vicia faba Leaves: IV. Site(s) of Fatty Acid Incorporation into the Major Glycerolipids.
机构信息
Department of Botany, University of Toronto, Ontario M5S 1A1 Canada.
出版信息
Plant Physiol. 1979 Apr;63(4):674-6. doi: 10.1104/pp.63.4.674.
The fatty acids of the major glycerolipids of Vicia faba leaves were analyzed immediately following (14)CO(2) feeding. The leaves were fractionated into chloroplast and cytoplasmic fractions and the location of radioactivity in the fatty acids determined. The results indicate that the major site of incorporation of fatty acids is in the phospholipids. Phosphatidylcholine contained the highest level of radioactivity in the cytoplasmic fraction, whereas phosphatidylglycerol contained radioactivity in both the chloroplast and cytoplasmic fractions. The galactolipids contained very little radioactivity in comparison, this radioactivity being confined to high speed centrifugal fractions believed to contain the envelopes of the chloroplast. Our results suggest that phosphatidylcholine is a major site of incorporation of fatty acids (mainly in oleic acid) in the cytoplasm, whereas phosphatidylglycerol is also a site of incorporation involving both oleic and palmitic acids, inside and outside the chloroplast.
立即分析（14）CO2 喂养后 Vicia faba 叶片中主要甘油磷脂的脂肪酸。将叶片分离为叶绿体和细胞质部分，并确定脂肪酸中放射性的位置。结果表明，脂肪酸掺入的主要部位是在磷脂中。细胞质部分的磷脂酰胆碱含有最高水平的放射性，而磷脂酰甘油在叶绿体和细胞质部分都含有放射性。与叶绿体和细胞质部分相比，半乳糖脂的放射性非常低，这种放射性仅限于被认为包含叶绿体包膜的高速离心部分。我们的结果表明，在细胞质中，磷脂酰胆碱是脂肪酸（主要是油酸）掺入的主要部位，而磷脂酰甘油也是一个涉及叶绿体内外的油酸和棕榈酸掺入的部位。
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