测定红光吸收型的光敏素的破坏。
Characterization of the Destruction of Phytochrome in the Red-absorbing Form.
机构信息
Department of Biology, Vanderbilt University, Nashville, Tennessee 37235.
出版信息
Plant Physiol. 1979 Apr;63(4):680-2. doi: 10.1104/pp.63.4.680.
Abstract
Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of dark-grown tissue, in 4-day-old etiolated oat seedlings. Pr and Pfr destruction follow the same time course, exhibit the same time delay after actinic irradiation when the plants are grown in sealed containers, result in a loss of antigenically detectable phytochrome, as determined by radial immunodiffusion assay, equal to the loss of spectrophotometrically detectable phytochrome, and have the same sensitivity to 2-mercaptoethanol and azide. We suggest that Pr destruction is a consequence of the same mechanism that is responsible for Pfr destruction.
摘要
在 4 天龄的暗培养燕麦幼苗中，红光吸收型（Pr）和远红光吸收型（Pfr）两种形式的光敏色素都发生光破坏，即黑暗生长组织经光照射后，光可逆检测到的色素蛋白丧失。Pr 和 Pfr 的破坏遵循相同的时间进程，当植物在密封容器中生长时，在光照射后具有相同的时间延迟，导致通过放射免疫扩散测定法检测到的抗原性可检测的光敏色素丧失，与分光光度法可检测到的光敏色素丧失相等，并且对 2-巯基乙醇和叠氮化钠的敏感性相同。我们认为，Pr 破坏是导致 Pfr 破坏的同一机制的结果。
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