Philips Research Laboratories, Eindhoven, The Netherlands.
Plant Physiol. 1979 Apr;63(4):765-8. doi: 10.1104/pp.63.4.765.
A method of phenol determination in plant leaves has been developed which is based on the in situ oxidation of these compounds in an atmosphere containing ammonia, followed by difference spectrophotometry. The development of the phenol pattern has been studied in each separate leaf of a Salvia occidentalis plant grown in short and in long days. During the light period the phenol content (mainly chlorogenic acid and isochlorogenic acids) increases in proportion to the length of this period, whereas during the subsequent dark period the phenol content decreases. This decrease does not continue during the second part of a dark period if that period is interrupted by a light break with red light. Instead a small increase is observed. This effect of red light can be reversed with far red light. It is argued that a correlation with flower induction in this short day plant can be construed if it is assumed that the continuous presence of certain o-dihydroxyphenols in the cytoplasm of leaf cells inhibits the synthesis or the transport of a flowering hormone.
已经开发出一种在含有氨的气氛中原位氧化这些化合物的植物叶片中苯酚测定方法，然后进行差示分光光度法。研究了在短日和长日条件下生长的西洋丹参植物的每片单独叶片中苯酚图谱的发展。在光期内，酚类物质（主要是绿原酸和异绿原酸）的含量与该期的长度成比例增加，而在随后的暗期中，酚类物质的含量减少。如果暗期的第二部分被红光的光中断打断，则不会继续减少。相反，观察到小的增加。这种红光的影响可以用远红光来逆转。如果假设细胞溶质中某些邻二羟基苯酚的持续存在抑制了开花激素的合成或运输，那么可以推断出与这种短日植物的花诱导之间存在相关性。
