棉籽及其他未发芽油籽中的苹果酸合酶活性：一项调查。
Malate synthase activity in cotton and other ungerminated oilseeds: a survey.
机构信息
Department of Botany and Microbiology, Arizona State University, Tempe, Arizona 85281.
出版信息
Plant Physiol. 1979 Jun;63(6):1068-71. doi: 10.1104/pp.63.6.1068.
Extracts from several species and varieties of ungerminated cotton seeds plus homogenates from 18 other oilseeds (representing 11 different families) were examined for malate synthase and isocitrate lyase activity. Malate synthase activities in the various cotton seeds ranged from 35 to 129% of the units per dry seed weight found in Deltapine 16 cotton. For other oilseeds, the range was from 0.3 to 58% of Deltapine 16 cotton. Castor bean (Ricinus communis L.) had the least activity per mg dry weight (12-fold lower than the next lowest species), while Pima cotton (Gossypium barbadense L.) had the highest level (8.53 units). On a per seed basis, these values were 15 and 747 nanomoles per minute.Malate synthase activity was measurable in all seed types examined, whereas isocitrate lyase could not be detected in any of the seeds. We suggest that synthesis of malate synthase during seed development is universal among oilseeds in the absence of glyoxylate-cycle-associated isocitrate lyase activity.
从几种未发芽的棉花种子和 18 种其他油籽（代表 11 个不同的科）的提取物以及匀浆中，检测了苹果酸合酶和异柠檬酸裂解酶的活性。各种棉花种子中的苹果酸合酶活性为每干种子重量单位的 35%至 129%，而在德尔塔品系 16 棉花中发现的则为每干种子重量单位的 35%至 129%。对于其他油籽，其范围为德尔塔品系 16 棉花的 0.3%至 58%。蓖麻（Ricinus communis L.）每毫克干重的活性最低（比下一个最低的物种低 12 倍），而皮马棉（Gossypium barbadense L.）的活性最高（8.53 个单位）。以种子为基础，这些值分别为 15 和 747 纳摩尔/分钟。在所有检查的种子类型中都可以测量到苹果酸合酶的活性，而在任何种子中都无法检测到异柠檬酸裂解酶。我们认为，在没有乙醛酸循环相关异柠檬酸裂解酶活性的情况下，苹果酸合酶在油籽发育过程中的合成是普遍存在的。
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