Department of Botany, The University of Sheffield, Sheffield S10 2TN, England.
Plant Physiol. 1979 Jun;63(6):1212-4. doi: 10.1104/pp.63.6.1212.
Intact protoplasts and chloroplasts have been isolated from mature flag leaves of wheat (Triticum aestivum L.). Both showed high rates of photosynthesis, the best of which equaled those observed in the parent tissue (greater than 150 micromoles O(2) per milligram chlorophyll per hour). The presence of ethylenediaminetetraacetate and an alkaline medium (pH 8.4) were required in the isolation and assay for the achievement of maximum rates of photosynthesis by chloroplasts. Photosynthesis by isolated chloroplasts was inhibited at very low concentrations of external orthophosphate.
已从成熟的小麦（Triticum aestivum L.）旗叶中分离出完整的原生质体和叶绿体。两者的光合速率都很高，最好的与亲本组织中观察到的相当（每小时每毫克叶绿素大于 150 微摩尔 O(2)）。在分离和测定叶绿体的最大光合速率时，需要乙二胺四乙酸和碱性介质（pH 8.4）。叶绿体分离后的光合作用在很低的外部正磷酸盐浓度下受到抑制。
