Casazza A P, Tarantino D, Soave C
Dept of Biology, University of Milano, via Celoria 26, 20133, Milano, Italy,
Photosynth Res. 2001;68(2):175-80. doi: 10.1023/A:1011818021875.
A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport chain, ATP/NADPH, ATP/O(2) and PS II/chlorophyll ratios. Sensitivity to a light-chill photoinhibitory treatment was also determined by evaluating the decrease in both maximal photochemical efficiency (Fv/Fm) and in electron transport rate.
开发了一种从拟南芥叶片中分离功能性类囊体的方案。获得活性、偶联且稳定制剂的关键因素是在研磨缓冲液中加入乙二胺四乙酸（EDTA）和乙二醇双四乙酸（EGTA）。根据整个或部分电子传递链、ATP/还原型辅酶Ⅱ（NADPH）、ATP/氧气（O₂）以及光系统Ⅱ/叶绿素比率对制剂进行了表征。还通过评估最大光化学效率（Fv/Fm）和电子传递速率的降低来确定对光冷光抑制处理的敏感性。
