Wagner G J
Department of Biology, Brookhaven National Laboratory, Upton, New York 11973.
Plant Physiol. 1979 Jul;64(1):88-93. doi: 10.1104/pp.64.1.88.
Neutral sugar, free amino acid, and anthocyanin levels and vacuole/extravacuole distribution were determined for Hippeastrum and Tulipa petal and Tulipa leaf protoplasts. Glucose and fructose, the predominant neutral monosaccharides observed, were primarily vacuolar in location. Glutamine, the predominant free amino acid found, was primarily extravacuolar. gamma-Methyleneglutamate was identified as a major constituent of Tulipa protoplasts. Qualitative characterization of Hippeastrum petal and vacuole organic acids indicated the presence of oxalic, malic, citric, and isocitric acids. Data are presented which indicate that vacuoles obtained by gentle osmotic shock of protoplasts in dibasic phosphate have good purity and retain their contents.
测定了朱顶红和郁金香花瓣以及郁金香叶片原生质体中的中性糖、游离氨基酸、花青素水平以及液泡/液泡外分布情况。观察到的主要中性单糖葡萄糖和果糖主要位于液泡中。所发现的主要游离氨基酸谷氨酰胺主要位于液泡外。γ-亚甲基谷氨酸被鉴定为郁金香原生质体的主要成分。对朱顶红花瓣和液泡有机酸的定性表征表明存在草酸、苹果酸、柠檬酸和异柠檬酸。所呈现的数据表明，通过在磷酸氢二盐中对原生质体进行温和渗透休克获得的液泡具有良好的纯度并保留其内含物。
