Section of Botany, Genetics and Development, Plant Science Building, Cornell University, Ithaca, New York 14853.
Plant Physiol. 1979 Aug;64(2):165-8. doi: 10.1104/pp.64.2.165.
The effect of a number of inhibitors on the ATP level in single cells of Chara corallina has been measured using the luciferin-luciferase assay. The uncouplers of phosphorylation, carbonyl cyanide m-chlorophenyl hydrazone and 2,4-dinitrophenol, and the ATPase inhibitors, dicyclohexyl-carbodimide and diethylstilbestrol, all caused a marked reduction of the ATP level. These inhibitors also produced a large increase in the membrane resistance and a depolarization of the membrane potential to the diffusion potential. This is consistent with the plasmalemma containing an ATP-dependent electrogenic pump that provides the primary conductance through the membrane.Ethyl-3-(3-dimethylaminopropyl)carbodiimide, which depolarizes the membrane potential, has no effect on the ATP level and does not increase the membrane resistance. This inhibitor apparently does not enter the cell but may act by affecting the permeability of the membrane. Neither darkness nor 3-(3,4-dichlorophenyl)-1,1-dimethylurea lowers the ATP level and, while neither has much effect on the membrane potential, both cause a similar increase in resistance in comparison with the control in the light. The weak acid, 5,5-dimethyloxazolidine-2,4-dione, and the weak base, NH(3), do not affect the ATP level significantly but have effects on the electrogenic pump that are consistent with their postulated effects on the cytoplasmic pH, if H(+) is the substrate for the pump.
已使用荧光素-荧光素酶检测法测量了几种抑制剂对单个珊瑚藻细胞中 ATP 水平的影响。解偶联磷酸化的羰基氰化物 m-氯苯腙和 2,4-二硝基苯酚，以及 ATP 酶抑制剂二环己基碳化二亚胺和己烯雌酚，均导致 ATP 水平显著降低。这些抑制剂还导致膜电阻显著增加，膜电位去极化至扩散电位。这与质膜含有依赖于 ATP 的电致泵一致，该泵为通过膜的主要电导提供动力。使膜电位去极化的乙基-3-(3-二甲基氨基丙基)碳化二亚胺对 ATP 水平没有影响，也不会增加膜电阻。这种抑制剂显然不会进入细胞，但可能通过影响膜的通透性起作用。黑暗或 3-(3,4-二氯苯基）-1,1-二甲基脲均不会降低 ATP 水平，虽然它们对膜电位的影响不大，但与在光下的对照相比，两者均导致电阻相似增加。弱酸 5,5-二甲基恶唑烷-2,4-二酮和弱碱 NH(3) 对 ATP 水平的影响不大，但对电致泵的影响与它们对细胞质 pH 的假设影响一致，如果 H(+) 是泵的底物。
