在液体培养基中培养的高等植物细胞内pH值的测量及其控制方面。

Measurement of intracellular pH and aspects of its control in higher plant cells cultivated in liquid medium.

作者信息

Kurkdjian A, Leguay J J, Guern J

出版信息

Respir Physiol. 1978 Apr;33(1):75-89. doi: 10.1016/0034-5687(78)90086-5.

Abstract

The 5,5-dimethyloxazolidine-2,4-dione (DMO) method for the determination of intracellular pH has been adapted to Acer pseudoplatanus cells cultivated in liquid medium. DMO is metabolized by these cells and this parameter has to be taken into account in the measurement of the intracellular concentration of free DMO. As the cells contain a large acidic vacuolar compartment, the intracellular pH estimated from the DMO technique is an overall pHi', the dependence of which on various cytoplasmic and vacuolar characteristics has been discussed. Important modifications of these overall pHi values under the influence of CO2 or NH4+ have been measured. These pHi modifications strongly affect the regulation of cell division through the control of the intracellular concentration of hormones of the auxin type.

摘要

用于测定细胞内pH值的5,5-二甲基恶唑烷-2,4-二酮（DMO）方法已适用于在液体培养基中培养的悬铃木细胞。DMO会被这些细胞代谢，在测量游离DMO的细胞内浓度时必须考虑这一参数。由于细胞含有一个大的酸性液泡区室，通过DMO技术估算的细胞内pH值是一个总体细胞内pH值（pHi'），已讨论了其对各种细胞质和液泡特性的依赖性。已测量了在CO2或NH4+影响下这些总体pHi值的重要变化。这些pHi变化通过控制生长素类激素的细胞内浓度强烈影响细胞分裂的调节。