Departments of Botany and Plant Pathology, Biological Sciences and Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1979 Sep;64(3):398-403. doi: 10.1104/pp.64.3.398.
Three independent methods, one cytological and two biochemical, were used to estimate contributions of plastids and plastid fragments to various membrane fractions. In thin sections viewed by electron microscopy, KMnO(4) selectively enhanced the images of plastid membranes in situ as well as in isolated fractions. The amounts of plastid fragments in isolated membrane fractions were determined by electron microscopic morphometry of fractions fixed with KMnO(4) in conjunction with analysis of galactolipids and carotenoids. Monogalactosyl and digalactosyl diglyceride contents were directly correlated with the amount of plastid membranes in the fractions identified by electron microscope morphometry. Amounts of carotenoids also correlated with plastid membranes except at very low levels where estimates based on carotenoids exceeded those based on morphometry.
采用三种独立的方法，一种细胞学方法和两种生化方法，来估计质体和质体片段对各种膜部分的贡献。在电子显微镜下观察的薄片中，KMnO4 选择性地增强了原位和分离部分中质体膜的图像。通过结合 KMnO4 固定的电子显微镜形态计量学分析半乳糖脂和类胡萝卜素，来确定分离膜部分中的质体片段的量。单半乳糖基和双半乳糖基二甘油酯的含量与电子显微镜形态计量学鉴定的部分中的质体膜的量直接相关。类胡萝卜素的含量也与质体膜相关，除了在非常低的水平下，基于类胡萝卜素的估计值超过基于形态计量学的估计值。
