Dörmann P, Hoffmann-Benning S, Balbo I, Benning C
Institut für Genbiologische Forschung Berlin GmbH, Germany.
Plant Cell. 1995 Nov;7(11):1801-10. doi: 10.1105/tpc.7.11.1801.
The galactolipids monogalactosyl and digalactosyl diacylglycerol occur in all higher plants and are the predominant lipid components of chloroplast membranes. They are thought to be of major importance to chloroplast morphology and physiology, although direct experimental evidence is still lacking. The enzymes responsible for final assembly of galactolipids are associated with the envelope membranes of plastids, and their biochemical analysis has been notoriously difficult. Therefore, we have chosen a genetic approach to study the biosynthesis and function of galactolipids in higher plants. We isolated a mutant of Arabidopsis that is deficient in digalactosyl diacylglycerol by directly screening a mutagenized M2 population for individuals with altered leaf lipid composition. This mutant carries a recessive nuclear mutation at a single locus designated dgd1. Backcrossed mutants show stunted growth, pale green leaf color, reduced photosynthetic capability, and altered thylakoid membrane ultrastructure.
半乳糖脂单半乳糖基二酰基甘油和二半乳糖基二酰基甘油存在于所有高等植物中，是叶绿体膜的主要脂质成分。尽管仍缺乏直接的实验证据，但它们被认为对叶绿体的形态和生理至关重要。负责半乳糖脂最终组装的酶与质体的包膜膜相关，对其进行生化分析一直非常困难。因此，我们选择了一种遗传学方法来研究高等植物中半乳糖脂的生物合成和功能。我们通过直接筛选诱变的M2群体中叶片脂质组成发生改变的个体，分离出了一个缺乏二半乳糖基二酰基甘油的拟南芥突变体。该突变体在一个名为dgd1的单一位点携带隐性核突变。回交突变体表现出生长发育迟缓、叶片淡绿色、光合能力下降以及类囊体膜超微结构改变。
