Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada.
Plant Physiol. 1980 Apr;65(4):587-90. doi: 10.1104/pp.65.4.587.
Shoot-forming tobacco callus is found to have high levels of adenosine phosphates and NAD(+), and a low energy charge during meristemoid and shoot primordium formation. NADH levels are low and show little change during this period. There is a decline in the content of NADPH to nondetectable levels during the process, and a transient increase of NADP(+) is observed early in culture. These patterns are indicative of a shift to a more intensive rate of metabolism during meristemoid and shoot primordium formation and apparently reflect the requirement for energy and reducing power during organ initiation.
在分生组织和芽原基形成过程中,发现芽生烟草愈伤组织具有高水平的腺嘌呤核苷酸和 NAD(+),以及低的能量电荷。在此期间,NADH 水平较低且变化不大。在这个过程中,NADPH 的含量下降到无法检测的水平,并且在培养早期观察到 NADP(+)的短暂增加。这些模式表明在分生组织和芽原基形成过程中代谢率的增加,显然反映了器官起始过程中对能量和还原力的需求。