在烟草愈伤组织培养中，在芽起始过程中的莽草酸途径活性。
Shikimate Pathway Activity during Shoot Initiation in Tobacco Callus Cultures.
机构信息
Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada.
出版信息
Plant Physiol. 1983 Oct;73(2):228-32. doi: 10.1104/pp.73.2.228.
Abstract
The activity of the shikimic acid pathway during shoot initiation in tobacco (Nicotiana tabacum L. Wisconsin 38) callus was examined. Enhancement of the activities of 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase, shikimate kinase, chorismate mutase, and anthranilate synthase was observed during culture of tobacco callus under shootforming conditions in comparison to tissue cultured under non-organforming conditions. Confirmation of these findings was obtained by examining the incorporation of d-[(14)C]glucose into quinic and shikimic acids and of [(14)C]shikimic acid into tyrosine, phenylalanine, and tryptophan.
摘要
在烟草（Nicotiana tabacum L. Wisconsin 38）愈伤组织的芽起始过程中，研究了莽草酸途径的活性。与非器官形成条件下的组织培养相比，在芽形成条件下培养烟草愈伤组织时，观察到 3-脱氧-d-阿拉伯庚酮糖 7-磷酸合酶、莽草酸激酶、分支酸变位酶和邻氨基苯甲酸合酶的活性增强。通过检查 d-[(14)C]葡萄糖掺入奎尼酸和莽草酸以及 [(14)C]莽草酸掺入酪氨酸、苯丙氨酸和色氨酸，证实了这些发现。
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