Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada.
Plant Physiol. 1983 Oct;73(2):228-32. doi: 10.1104/pp.73.2.228.
The activity of the shikimic acid pathway during shoot initiation in tobacco (Nicotiana tabacum L. Wisconsin 38) callus was examined. Enhancement of the activities of 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase, shikimate kinase, chorismate mutase, and anthranilate synthase was observed during culture of tobacco callus under shootforming conditions in comparison to tissue cultured under non-organforming conditions. Confirmation of these findings was obtained by examining the incorporation of d-[(14)C]glucose into quinic and shikimic acids and of [(14)C]shikimic acid into tyrosine, phenylalanine, and tryptophan.
在烟草(Nicotiana tabacum L. Wisconsin 38)愈伤组织的芽起始过程中,研究了莽草酸途径的活性。与非器官形成条件下的组织培养相比,在芽形成条件下培养烟草愈伤组织时,观察到 3-脱氧-d-阿拉伯庚酮糖 7-磷酸合酶、莽草酸激酶、分支酸变位酶和邻氨基苯甲酸合酶的活性增强。通过检查 d-[(14)C]葡萄糖掺入奎尼酸和莽草酸以及 [(14)C]莽草酸掺入酪氨酸、苯丙氨酸和色氨酸,证实了这些发现。