Centro di Studio del Consiglio Nazionale delle Ricerche per la Biologia Cellulare e Molecolare delle Piante, Istituto di Scienze Botaniche, Via Giuseppe Colombo 60, 20133 Milan, Italy.
Plant Physiol. 1981 Feb;67(2):212-5. doi: 10.1104/pp.67.2.212.
A mild tryptic digestion of chloroplast membranes eliminates the effects of saturating concentrations of cations (3 to 5 millimolar MgCl(2)) on chlorophyll fluorescence yield, membrane stacking, and photosystem II photochemical efficiency in spinach. At the same time, the negative surface potential of the membranes is increased (by trypsin) as revealed by studies with 9-aminoacridine. High concentrations of cations (25 to 100 millimolar MgCl(2)) added after trypsin digestion are effective in restoring high fluorescence yields and membrane stacking. High concentrations of cations added after trypsin treatment do not increase the photosystem II efficiency. It is concluded that the "diffuse electrical layer" hypothesis of Barber et al. (Barber J, J Mills, A Love, 1977 FEBS Lett 74: 174-181) satisfactorily explains the effect of trypsin in eliminating the influence of saturating concentrations of cations on chlorophyll fluorescence yield and membrane stacking. However, the effect on photosystem II photochemical efficiency seems to require another mechanism.
温和的胰蛋白酶消化叶绿体膜可以消除饱和浓度阳离子（3 至 5 毫摩尔 MgCl2）对叶绿素荧光产量、膜堆叠和菠菜中光系统 II 光化学效率的影响。同时，通过 9-氨基吖啶的研究表明，膜的负表面电势（由胰蛋白酶）增加。胰蛋白酶消化后添加高浓度阳离子（25 至 100 毫摩尔 MgCl2）可有效恢复高荧光产量和膜堆叠。胰蛋白酶处理后添加高浓度阳离子不会增加光系统 II 的效率。因此，可以得出结论，巴伯等人的“弥散电场层”假说（巴伯 J、J 米尔斯、A 洛夫，1977 年 FEBS Lett 74：174-181）可以很好地解释胰蛋白酶在消除饱和浓度阳离子对叶绿素荧光产量和膜堆叠的影响方面的作用。然而，对光系统 II 光化学效率的影响似乎需要另一种机制。
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