生产并鉴定针对核糖基玉米素的抗体，并建立放射免疫分析法。
Production and characterization of antibodies and establishment of a radioimmunoassay for ribosylzeatin.
机构信息
Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque, New Mexico 87131.
出版信息
Plant Physiol. 1981 Mar;67(3):401-3. doi: 10.1104/pp.67.3.401.
An antibody directed towards ribosyl-trans-zeatin has been produced and characterized. The antiserum was produced in rabbits using ribosyl-zeatin-bovine serum albumin as an immunogen. A radioimmunoassay which employed this antiserum and a tritiated antigen was established. As little as 10 picomoles ribosyl-trans-zeatin could be detected. The specificity of the antiserum was measured in the radioimmunoassay by using nonradioactive nucleosides as competitive inhibitors. Changes in position N(6) were more effective in decreasing antibody recognition than changes in position 2. Of particular interest was the interaction of the isomer ribosyl-cis-zeatin. This compound was significantly less active as an inhibitor than ribosyl-trans-zeatin, demonstrating that the antibody was sensitive to minor changes in the structure of the antigen.The use of this antibody and the radioimmunoassay for ribosylzeatin provides a rapid method for the detection of ribosylzeatin, as well as offering the potential for immunoadsorbent columns which would be useful in the purification of macromolecules, such as tRNA, which contain the ribosylzeatin moiety.
一种针对核糖基反式玉米素的抗体已经被制备和鉴定。该抗血清是用核糖基玉米素-牛血清白蛋白作为免疫原在兔子中产生的。建立了一种放射免疫测定法，该方法使用这种抗血清和氚标记抗原。只需 10 皮摩尔的核糖基反式玉米素就可以被检测到。该抗血清的特异性在放射免疫测定中通过使用非放射性核苷作为竞争性抑制剂来测量。位置 N(6)的变化比位置 2 的变化更能有效地降低抗体的识别。特别有趣的是顺式玉米素异构体的相互作用。与核糖基反式玉米素相比，该化合物作为抑制剂的活性显著降低，这表明抗体对抗原结构的微小变化很敏感。该抗体和玉米素的放射免疫测定法的使用为玉米素的检测提供了一种快速的方法，同时也为含有玉米素部分的 tRNA 等大分子的纯化提供了有用的免疫吸附柱。
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