马铃薯块茎形成的生理学研究：马铃薯植株中的顺式玉米素核苷：其鉴定及在温度和光周期影响下的内源水平变化。
Physiology of Tuberization in Solanum tuberosum L: cis-Zeatin Riboside in the Potato Plant: Its Identification and Changes in Endogenous Levels as Influenced by Temperature and Photoperiod.
机构信息
Department of Plant and Soil Sciences, University of Maine, Orono, Maine 04473.
出版信息
Plant Physiol. 1978 Sep;62(3):438-42. doi: 10.1104/pp.62.3.438.
Using high pressure liquid chromatography, the cucumber cotyledon bioassay, and mass spectrometry a cytokinin isolated from Solanum tuberosum L. cv. Katahdin plant tissues has been identified as cis-zeatin riboside. Zeatin riboside (ZR) levels in plants grown under inducing conditions (28 C day and 13 C night with a 10-hour photoperiod) were significantly higher than those in plants grown under noninducing conditions (30 C day and 28 C night with an 18-hour photoperiod). The highest level of ZR was noted in below-ground tissue after 4 days exposure to inducing conditions, with tuber initiation observed after 8 days. A companion study conducted to determine the effect of ZR on in vitro tuberization of noninduced rhizomes revealed that after 1 month in culture, controls exhibited 0% tuberization, while ZR treatments of 0.3 and 3.0 milligrams per liter showed 39 and 75% tuberization, respectively.
使用高压液相色谱法、黄瓜子叶生物测定法和质谱法，从马铃薯品种 Katahdin 的植物组织中分离出一种细胞分裂素，被鉴定为顺式玉米素核苷。在诱导条件（28°C 白天和 13°C 夜间，10 小时光周期）下生长的植物中的玉米素核苷（ZR）水平明显高于在非诱导条件（30°C 白天和 28°C 夜间，18 小时光周期）下生长的植物。在 4 天暴露于诱导条件后，地下组织中观察到最高水平的 ZR，8 天后观察到块茎起始。进行了一项伴随的研究，以确定 ZR 对非诱导根状茎体外结薯的影响，结果表明，在培养 1 个月后，对照中无结薯，而 ZR 处理 0.3 和 3.0 毫克/升分别显示出 39%和 75%的结薯率。
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