Department of Plant and Soil Sciences, University of Maine, Orono, Maine 04473.
Plant Physiol. 1978 Sep;62(3):438-42. doi: 10.1104/pp.62.3.438.
Using high pressure liquid chromatography, the cucumber cotyledon bioassay, and mass spectrometry a cytokinin isolated from Solanum tuberosum L. cv. Katahdin plant tissues has been identified as cis-zeatin riboside. Zeatin riboside (ZR) levels in plants grown under inducing conditions (28 C day and 13 C night with a 10-hour photoperiod) were significantly higher than those in plants grown under noninducing conditions (30 C day and 28 C night with an 18-hour photoperiod). The highest level of ZR was noted in below-ground tissue after 4 days exposure to inducing conditions, with tuber initiation observed after 8 days. A companion study conducted to determine the effect of ZR on in vitro tuberization of noninduced rhizomes revealed that after 1 month in culture, controls exhibited 0% tuberization, while ZR treatments of 0.3 and 3.0 milligrams per liter showed 39 and 75% tuberization, respectively.
使用高压液相色谱法、黄瓜子叶生物测定法和质谱法，从马铃薯品种 Katahdin 的植物组织中分离出一种细胞分裂素，被鉴定为顺式玉米素核苷。在诱导条件（28°C 白天和 13°C 夜间，10 小时光周期）下生长的植物中的玉米素核苷（ZR）水平明显高于在非诱导条件（30°C 白天和 28°C 夜间，18 小时光周期）下生长的植物。在 4 天暴露于诱导条件后，地下组织中观察到最高水平的 ZR，8 天后观察到块茎起始。进行了一项伴随的研究，以确定 ZR 对非诱导根状茎体外结薯的影响，结果表明，在培养 1 个月后，对照中无结薯，而 ZR 处理 0.3 和 3.0 毫克/升分别显示出 39%和 75%的结薯率。
