Department of Pomology, University of California, Davis, CA 95616.
Plant Physiol. 1981 Oct;68(4):823-6. doi: 10.1104/pp.68.4.823.
Pear fruit cells undergoing a period of senescence in auxin-deprived media develop a substantial cyanide resistant respiration in response to the addition of 0.7 to 3.5 micromolar cycloheximide. The inhibitor does not affect overall cellular repiratory activity and titrations with salicylhydroxamic acid reveal that only a minor portion, about 10%, of the alternate pathway is utilized by the cycloheximide-treated senescent cells. The alternate respiratory pathway appears to be of mitochondrial origin but is not induced by chloramphenicol.
在不含生长素的培养基中，鸭梨果实细胞衰老时，若添加 0.7 至 3.5 微摩尔环己酰亚胺，会产生大量氰化物抗性呼吸。该抑制剂不影响细胞的总呼吸活性，用水杨酸羟肟酸滴定表明，环己酰亚胺处理的衰老细胞只利用交替途径的一小部分，约 10%。该交替呼吸途径似乎来自线粒体，但氯霉素不能诱导。
