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烟草原生质体有丝分裂发育的激素控制：新合成蛋白质的二维分布

Hormonal Control of Mitotic Development in Tobacco Protoplasts: TWO-DIMENSIONAL DISTRIBUTION OF NEWLY-SYNTHESIZED PROTEINS.

作者信息

Meyer Y, Chartier Y

机构信息

Laboratoire de Physiologie Végétale, Université de Perpignan, Avenue de Villeneuve, F-66025 Perpignan-Cedex, France.

出版信息

Plant Physiol. 1981 Dec;68(6):1273-8. doi: 10.1104/pp.68.6.1273.

DOI:10.1104/pp.68.6.1273
PMID:16662091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC426086/
Abstract

Two-dimensional separation of proteins newly synthesized by tobacco mesophyll protoplasts cultivated in vitro allows us to detect, reproducibly, 257 spots. The pattern is extremely stable throughout the three days of culture, the intensity of only 24 spots varying during this time. The absence of cytokinin (N(6)-benzyladenine) in the culture medium prohibits entry into S phase but does not modify the pattern, indicating that none of the observed proteins is specifically synthesized in S, G(2), or M phases. The presence of 2,4-dichlorophenoxyacetic acid is necessary for the mitotic development of protoplasts. It induces the appearance of one protein, increases the level of another, and reduces that of eight others. All proteins sensitive to auxin belong to the group of proteins the levels of which vary during culture.

摘要

对体外培养的烟草叶肉原生质体新合成的蛋白质进行二维分离，使我们能够重复检测到257个斑点。在整个三天的培养过程中，该模式极其稳定，在此期间只有24个斑点的强度发生变化。培养基中缺乏细胞分裂素（N⁶-苄基腺嘌呤）会阻止进入S期，但不会改变该模式，这表明所观察到的蛋白质中没有一种是在S期、G₂期或M期特异性合成的。2,4-二氯苯氧乙酸的存在对原生质体的有丝分裂发育是必要的。它诱导一种蛋白质的出现，提高另一种蛋白质的水平，并降低其他八种蛋白质的水平。所有对生长素敏感的蛋白质都属于在培养过程中水平会发生变化的蛋白质组。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/ed04444c79bb/plntphys00542-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/ae31c5310a66/plntphys00542-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/799c059dc22b/plntphys00542-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/ed04444c79bb/plntphys00542-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/ae31c5310a66/plntphys00542-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/799c059dc22b/plntphys00542-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05db/426086/ed04444c79bb/plntphys00542-0073-a.jpg

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Hormonal Control of Mitotic Development in Tobacco Protoplasts: TWO-DIMENSIONAL DISTRIBUTION OF NEWLY-SYNTHESIZED PROTEINS.烟草原生质体有丝分裂发育的激素控制：新合成蛋白质的二维分布
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本文引用的文献

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Effect of anoxia on energy charge and protein synthesis in rice embryo.缺氧对水稻胚能量状态和蛋白质合成的影响。
Plant Physiol. 1981 Sep;68(3):636-40. doi: 10.1104/pp.68.3.636.
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培养的烟草组织中的一种可溶的生长素结合蛋白可刺激体外的 RNA 合成。
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Purification, immunoassay and characterization of an abundant, cytokinin-regulated polypeptide in cultured tobacco tissues : Evidence the protein is a β-1,3-glucanase.培养烟草组织中丰富的细胞分裂素调节多肽的纯化、免疫测定和特性：该蛋白是β-1,3-葡聚糖酶的证据。
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Patterns of protein synthesis in dividing and auxin-starved soybean cell suspensions. Differential expression of a major group of Mr-17000 peptides.分裂和生长素饥饿的大豆细胞悬浮液中的蛋白质合成模式。Mr-17000 肽主要组别的差异表达。
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Phase-Specific Polypeptides and Poly(A) RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells.细胞周期同步培养的长春花细胞中阶段性多肽和多聚（A）RNA。
Plant Physiol. 1989 Mar;89(3):910-7. doi: 10.1104/pp.89.3.910.
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Protein synthesis associated with quiescence and senescence in auxin-starved pear cells.饥饿态生长素处理下梨细胞中静止和衰老相关的蛋白质合成。
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Regulation of Gene Expression in Developing Zea mays Embryos: Protein Synthesis during Embryogenesis and Early Germination of Maize.玉米胚胎发育过程中的基因表达调控：胚胎发生和玉米早期萌发过程中的蛋白质合成。
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Developmental regulation of the synthesis of proteins encoded by stored mRNA in radish embryos.贮藏 mRNA 编码的蛋白质在萝卜胚胎中的合成的发育调控。
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A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
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Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.通过荧光自显影对聚丙烯酰胺凝胶中的³H和¹⁴C进行定量胶片检测。
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The glucocorticoid domain: steroid-mediated changes in the rate of synthesis of rat hepatoma proteins.糖皮质激素结构域：类固醇介导的大鼠肝癌蛋白合成速率变化
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