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香蕉果实成熟过程中的差异蛋白积累。

Differential Protein Accumulation in Banana Fruit during Ripening.

机构信息

Departamento de Biologia Molecular y Agrobiologia, Centro de Investigación y Desarrollo, Consejo Superior de Investigaciones Científicas, Jordi Girona Salgado 18-26, 08034 Barcelona, Spain.

出版信息

Plant Physiol. 1992 Jan;98(1):157-62. doi: 10.1104/pp.98.1.157.

DOI:10.1104/pp.98.1.157
PMID:16668607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1080163/
Abstract

Banana (Musa acuminata, cv Dwarf Cavendish) proteins were extracted from pulp tissue at different stages of ripening and analyzed by two-dimensional electrophoresis. The results provide evidence of differential protein accumulation during ripening. Two sets of polypeptides have been detected that increase substantially in ripe fruit. These polypeptides were characterized as glycoproteins by western blotting and concanavalin A binding assays. Antibodies againts tomato polygalacturonase cross-react with one of these sets of proteins.

摘要

香蕉（Musa acuminata，cv Dwarf Cavendish）蛋白质从不同成熟阶段的果肉组织中提取，并通过二维电泳进行分析。结果提供了在成熟过程中差异蛋白质积累的证据。已经检测到两组显著增加的多肽。这些多肽通过 Western 印迹和伴刀豆球蛋白 A 结合测定法被鉴定为糖蛋白。抗番茄多聚半乳糖醛酸酶的抗体与其中一组蛋白质发生交叉反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/0dff5300bc70/plntphys00700-0176-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/b65aa349ae5c/plntphys00700-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/ea294b774942/plntphys00700-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/c9d41b34b9db/plntphys00700-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/0dff5300bc70/plntphys00700-0176-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/b65aa349ae5c/plntphys00700-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/ea294b774942/plntphys00700-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/c9d41b34b9db/plntphys00700-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5db/1080163/0dff5300bc70/plntphys00700-0176-b.jpg

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本文引用的文献

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