用暗培养的大麦叶片将 5-氨基乙酰丙酸分解为 CO2
Catabolism of 5-Aminolevulinic Acid to CO(2) by Etiolated Barley Leaves.
机构信息
Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
出版信息
Plant Physiol. 1982 Jan;69(1):19-22. doi: 10.1104/pp.69.1.19.
The in vivo oxidation of the C(4) and C(5) of 5-aminolevulinic acid (ALA) to CO(2) has been studied in etiolated barley (Hordeum vulgare L. var. Larker) leaves in darkness. The rate of (14)CO(2) evolution from leaves fed [4-(14)C]ALA is strongly inhibited by aminooxyacetate, anaerobiosis, and malonate. The rate of (14)CO(2) evolution from leaves fed [5-(14)C]ALA is also inhibited by these treatments but to a lesser extent. These results suggest that (a) one step in ALA catabolism is a transamination reaction and (b) the C(4) is oxidized to CO(2) via the tricarboxylic acid cycle to a greater extent than is the C(5).
在黑暗条件下，对暗培养大麦（Hordeum vulgare L. var. Larker）叶片中 5-氨基乙酰丙酸（ALA）的 C(4)和 C(5)的体内氧化作用进行了研究。用[4-(14)C]ALA 喂养的叶片中 (14)CO(2)的释放速率受到氨基氧乙酸、无氧条件和丙二酸盐的强烈抑制。用[5-(14)C]ALA 喂养的叶片中 (14)CO(2)的释放速率也受到这些处理的抑制，但程度较轻。这些结果表明：（a）ALA 分解代谢的一个步骤是转氨反应；（b）C(4)通过三羧酸循环被氧化为 CO(2)的程度大于 C(5)。
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