Ravanel P, Tissut M, Douce R
Laboratoire de Physiologie Cellulaire Végétale, Université Scientifique et Médicale de Grenoble, B. P. 53X-38041, Grenoble, Cedex, France.
Plant Physiol. 1982 Feb;69(2):375-8. doi: 10.1104/pp.69.2.375.
The effects of kaempferol on the oxidative and phosphorylative properties of plant mitochondria from potato tubers and etiolated mung bean (Phaseolus aureus Roxb.) hypocotyls were investigated. Kaempferol inhibited the state 3 oxidation rate of malate, NADH, and succinate, but was without effect on the ascorbate-tetramethyl p-phenylenediamine oxidation rate. The inhibition was almost the same whether the mitochondria were in state 3 or in an uncoupled state 3. When 180 micromolar kaempferol was added during state 4, the tight coupling of succinate or NADH oxidation was not released. The results obtained indicate that kaempferol inhibits the mitochondrial electron flow at, or just after, the flavoprotein site.
研究了山奈酚对马铃薯块茎和黄化绿豆(Phaseolus aureus Roxb.)下胚轴植物线粒体氧化和磷酸化特性的影响。山奈酚抑制了苹果酸、NADH和琥珀酸的状态3氧化速率,但对抗坏血酸-四甲基对苯二胺氧化速率没有影响。无论线粒体处于状态3还是解偶联的状态3,抑制作用几乎相同。在状态4期间加入180微摩尔山奈酚时,琥珀酸或NADH氧化的紧密偶联并未解除。所得结果表明,山奈酚在黄素蛋白位点或其之后抑制线粒体电子流。