Department of Plant Pathology, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1983 May;72(1):151-5. doi: 10.1104/pp.72.1.151.
The glyceollin inhibition of electron transport by isolated soybean and corn mitochondria was similar to that of rotenone, acting at site I between the internal NADH dehydrogenase and coenzyme Q. Coupled state 3 malate oxidation was inhibited by glyceollin and rotenone with apparent K(i) values of about 15 and 5 micromolar, respectively. Carbonylcyanide m-chlorophenyl hydrazone uncoupled state 4 malate oxidation was also inhibited by glyceollin and rotenone, but uncoupled succinate and exogenous NADH state 4 oxidation was only slightly inhibited by both compounds. Glyceollin also inhibited ferricyanide reduction with malate as the electron donor, with an apparent K(i) of 5.4 micromolar, but failed to inhibit such reduction with succinate or externally added NADH as electron donors. Glyceollin did not inhibit state 4 oxidation of malate, succinate, or exogenous NADH. Glyceollin did not act as a classical uncoupler or as an inhibitor of oxidative phosphorylation.
大豆和玉米线粒体分离物中，glyceollin 对电子传递的抑制作用类似于鱼藤酮，作用部位在 NADH 脱氢酶和辅酶 Q 之间的内部 I 位点。偶联状态 3 苹果酸氧化被 glyceollin 和鱼藤酮抑制，其表观 K(i) 值分别约为 15 和 5 微摩尔。羰基氰化物 m-氯苯腙解偶联状态 4 苹果酸氧化也被 glyceollin 和鱼藤酮抑制，但两种化合物对解偶联的琥珀酸和外源 NADH 状态 4 氧化的抑制作用较小。glyceollin 也抑制铁氰化物还原，以苹果酸作为电子供体，其表观 K(i) 值为 5.4 微摩尔，但不能抑制琥珀酸或外加 NADH 作为电子供体的这种还原。glyceollin 不抑制苹果酸、琥珀酸或外源 NADH 的状态 4 氧化。glyceollin 不作为经典解偶联剂或氧化磷酸化抑制剂起作用。
