Racusen R H, Cooke T J
Department of Botany, University of Maryland, College Park, Maryland 20742.
Plant Physiol. 1982 Aug;70(2):331-4. doi: 10.1104/pp.70.2.331.
Electrophysiological procedures were used to evaluate cellular responses of fern (Onoclea sensibilis L.) gametophytes to photomorphogenetically active light. Red, far red, and blue light caused rapid changes in the membrane potential of the apical cell of the gametophyte filament; other cells in the filament were not similarly responsive. Measurements made with one electrode in the apical cell revealed that the membrane potential depolarized in red light and repolarized in far red light. Irradiation with blue light caused a hyperpolarization, the rapidity of which was dependent on a red light pretreatment. More refined measurements with one electrode in the tip of the apical cell and another in the base of the cell showed that both red and blue light treatments cause the apical cell to behave as a dipole. Because of the profound, long-term morphological changes that follow light irradiation in this organism, it was hoped to use it to elucidate the role that electrical parameters play in determining subsequent developmental events.
采用电生理方法评估蕨类植物（敏感鳞毛蕨）配子体对光形态建成活性光的细胞反应。红光、远红光和蓝光会使配子体丝状体顶端细胞的膜电位迅速发生变化；丝状体中的其他细胞则没有类似反应。用一个电极在顶端细胞中进行测量发现，膜电位在红光下 depolarized，在远红光下 repolarized。蓝光照射会导致超极化，其速度取决于红光预处理。用一个电极在顶端细胞尖端、另一个电极在细胞基部进行更精细的测量表明，红光和蓝光处理都会使顶端细胞表现为偶极子。由于该生物体在光照后会发生深刻的长期形态变化，因此希望利用它来阐明电参数在决定后续发育事件中所起的作用。
