Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiol. 1982 Sep;70(3):922-5. doi: 10.1104/pp.70.3.922.
The tissue distributions of cholorgenic acid, chlorogenic acid oxidase, and three other enzymes involved in the metabolism of this secondary (natural) product have been investigated in leaf-blades of light-grown seedlings of Sorghum bicolor. Cholorogenic acid was found only in epidermal and mesophyll protoplasts isolated from the leaf; 60% of the chlorogenic was contained in the epidermal fraction. Nearly all (90%) of the chlorogenic acid oxidase was found in the mesophyll protoplasts. The bundle-sheath strands, on the other hand, contained no chlorogenic acid and essentially none of the oxidase. Three other enzymes required for the synthesis of chlorogenic acid, but also for other plant products, were found in all three tissue fractions.
在光培养的高粱叶片中，研究了胆源性酸、胆源性酸氧化酶以及其他三种参与这种次生（天然）产物代谢的酶在组织中的分布。胆源性酸仅存在于从叶片中分离出的表皮和叶肉原生质体中；60%的胆源性酸存在于表皮部分。几乎所有（90%）的胆源性酸氧化酶存在于叶肉原生质体中。另一方面，束鞘束中不含胆源性酸，也基本上不含氧化酶。另外三种合成胆源性酸所需的酶，但也用于其他植物产品，在所有三个组织部分中都有发现。
