β-内酰胺基tabtoxin对豌豆谷氨酰胺合成酶的抑制作用
Inhibition of Glutamine Synthetase from Pea by Tabtoxinine-beta-lactam.
作者信息
Thomas M D, Langston-Unkefer P J, Uchytil T F, Durbin R D
机构信息
Department of Plant Pathology, University of Wisconsin-Madison and United States Department of Agriculture, Agricultural Research Service.
出版信息
Plant Physiol. 1983 Apr;71(4):912-5. doi: 10.1104/pp.71.4.912.
Abstract
Tabtoxinine-beta-lactam, a hydrolytic product of tabtoxin produced by Pseudomonas syringae pv. tabaci, apparently inactivates pea seed glutamine synthetase. Inhibition of the enzyme's initial velocity is linear over a range of 0.5 to 5 millimolar tabtoxinine-beta-lactam in the presence of 10 millimolar glutamate. A method for the purification of glutamine synthetase from dried peas is presented which gives a 30% yield with a 2,000-fold increase in specific activity. A method for obtaining highly purified tabtoxinine-beta-lactam and tabtoxin in good yields is also presented. The authenticity and purity of tabtoxinine-beta-lactam and tabtoxin were verified by chromatography, biological activity, and (1)H and (13)C nuclear magnetic resonance spectroscopy.
摘要
烟草毒素β-内酰胺是丁香假单胞菌烟草致病变种产生的烟草毒素的水解产物，它显然能使豌豆种子谷氨酰胺合成酶失活。在10毫摩尔谷氨酸存在的情况下，在0.5至5毫摩尔烟草毒素β-内酰胺范围内，该酶初始速度的抑制呈线性关系。本文介绍了一种从干豌豆中纯化谷氨酰胺合成酶的方法，该方法产率为30%，比活性提高了2000倍。还介绍了一种能高产率获得高纯度烟草毒素β-内酰胺和烟草毒素的方法。通过色谱法、生物活性以及氢-1和碳-13核磁共振光谱对烟草毒素β-内酰胺和烟草毒素的真实性和纯度进行了验证。
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