Laboratoire de Physiologie Végétale, Centre de Recherche de Bordeaux, 33140 Pont de la Maye, France.
Plant Physiol. 1983 May;72(1):115-21. doi: 10.1104/pp.72.1.115.
Rice (Oryza sativa var. Cigalon) is a plant which can adapt to very stringent anoxic conditions. It has previously been shown that during the adaptation period the energy charge can be used as a marker for metabolic activity. We have studied RNA metabolism during this period and correlated it with changes in the energy charge. Uptake of labeled precursor, UTP-specific activity, and incorporation were measured. Immediately after transfer to anaerobic conditions, the UTP pool size is reduced and the overall rate of incorporation drops. During adaptation, the rate of incorporation increases and stabilizes at about half of its value in aerobic conditions. Analysis of RNA shows that rRNA and mRNA are synthesized and that the processing of ribosomal RNA precursor is altered. Polyribosomes are present throughout the adaptation period although their amount is reduced during the first hour of anoxia. Changes in poly(A) content were noticed, indicating that some mRNA are rapidly degraded. Taken together, the results show that the RNA metabolism can be modulated during adaptation to anoxia in a parallel manner with energy charge changes.
水稻（Oryza sativa var. Cigalon）是一种能够适应非常严格的缺氧条件的植物。先前已经表明，在适应期内，能量电荷可以用作代谢活性的标志物。我们在此期间研究了 RNA 代谢，并将其与能量电荷的变化相关联。测量了标记前体的摄取、UTP 比活度和掺入。转移到厌氧条件后，UTP 池的大小会减少，整体掺入率会下降。在适应过程中，掺入率增加并稳定在有氧条件下的一半左右。RNA 分析表明 rRNA 和 mRNA 被合成，并且核糖体 RNA 前体的加工发生改变。多核糖体存在于整个适应期，尽管在缺氧的第一个小时内其数量会减少。多聚腺苷酸含量的变化表明一些 mRNA 迅速降解。总之，这些结果表明，在适应缺氧过程中，RNA 代谢可以与能量电荷变化平行调节。
