Ferl R J, Brennan M D, Schwartz D
Biochem Genet. 1980 Aug;18(7-8):681-91. doi: 10.1007/BF00484585.
Total cellular RNA from anaerobically stressed maize seedling roots was used to stimulate in vitro translation of authentic maize alcohol dehydrogenase (ADH) in a rabbit reticulocyte lysate system. Total products from such reactions were displayed on NEPHGE-SDS two-dimensional gels and the Adhl-specific translation products were identified by using RNA from sib seedlings segregating for Adhl charge and size variants. The application of a rapid RNA isolation procedure allowed the efficient isolation of biologically active RNAs from small amonts of seedling material. Maize ADHs translated in vitro are identical in size to in vivo ADH. Further, no ADH was detected in the products of an in vitro translation reaction stimulated by total RNA from aerobically grown seedlings. This suggests that induction of ADH protein by anaerobic stress is accomplished by production of Adh mRNA rather than activation of sequestered mRNA. The mRNAs for maize ADH1 and ADH2 are among a small class of mRNAs induced during anaerobiosis.
来自厌氧胁迫玉米幼苗根的总细胞RNA用于在兔网织红细胞裂解物系统中刺激天然玉米醇脱氢酶（ADH）的体外翻译。此类反应的总产物在非平衡pH梯度-十二烷基硫酸钠二维凝胶上展示，通过使用来自分离Adh电荷和大小变体的同胞幼苗的RNA来鉴定Adh1特异性翻译产物。快速RNA分离程序的应用使得能够从小量幼苗材料中高效分离出生物活性RNA。体外翻译的玉米ADH在大小上与体内ADH相同。此外，由需氧生长幼苗的总RNA刺激的体外翻译反应产物中未检测到ADH。这表明厌氧胁迫对ADH蛋白的诱导是通过Adh mRNA的产生而非封存mRNA的激活来实现的。玉米ADH1和ADH2的mRNA属于厌氧期间诱导的一小类mRNA。
