Schumacher T E, Smucker A J
Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1114.
Plant Physiol. 1983 May;72(1):212-4. doi: 10.1104/pp.72.1.212.
Total dissolved inorganic carbon (SigmaCO(2)) and aqueous carbon dioxide (H(2)CO(3) ()) in nutrient solutions may be measured by the injection of small gas or liquid samples (1 microliter to 8 milliliters) into a gas stripping column connected in-line with an infrared gas analyzer. The measurement of SigmaCO(2) in solution requires sample acidification, while H(2)CO(3) () and gaseous CO(2) are measured without the addition of lactic acid. The standard curve for SigmaCO(2) was linear up to 300 nanomoles CO(2). Maximum sensitivity was approximately 300 picomoles. Measurements of H(2)CO(3) () were independent of pH. Consequently, SigmaCO(2) and H(2)CO(3) () could be used to calculate the pH, HCO(3) (-), and CO(3) (2-) values of nutrient solutions. Injection and complete analyses required from 0.8 to 2 minutes.
营养液中的总溶解无机碳（SigmaCO₂）和二氧化碳水溶液（H₂CO₃()）可通过将小体积气体或液体样品（1微升至8毫升）注入与红外气体分析仪在线连接的气体吹扫柱来测量。溶液中SigmaCO₂的测量需要对样品进行酸化，而H₂CO₃()和气态CO₂的测量则无需添加乳酸。SigmaCO₂的标准曲线在高达300纳摩尔CO₂时呈线性。最大灵敏度约为300皮摩尔。H₂CO₃()的测量与pH无关。因此，SigmaCO₂和H₂CO₃()可用于计算营养液的pH、HCO₃(-)和CO₃(2-)值。进样和完整分析需要0.8至2分钟。
