Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Plant Physiol. 1983 Aug;72(4):1084-7. doi: 10.1104/pp.72.4.1084.
Conditions are described whereby suspensions of Chlorella pyrenoidosa and Netrium digitus photosynthetically biosynthesize and excrete glycolate continuously in high yields. Aminooxyacetic acid, an inhibitor of pyridoxal phosphate-linked enzymes, increased the excretion of glycolate approximately 4-fold in 1 hour (8 millimolar) and 20-fold in 4 hours (40 millimolar) in the presence of 0.2% CO(2) in air. The amount of glycolate excreted in the presence of aminooxyacetate and an atmosphere of 0.2% CO(2) in air equaled or exceeded the amount excreted in 0.2% CO(2) in O(2) minus aminooxyacetate. CO(2) and light were required for glycolate excretion. Aminooxyacetate also stimulated photosynthetic glycolate excretion in an atmosphere of 0.2% CO(2) in nitrogen or helium, although the stimulation was not as great as when air or O(2) was present.The excreted glycolate was converted to H(2) and CO(2) by the combined action of glycolic oxidase and the formic hydrogenlyase complex found in Escherichia coli in total conversion yields of 80%.
描述了条件下的小球藻和鱼腥藻 photosynthetically 生物合成和排泄不断在高产量的乙二醇。氨氧乙酸，抑制剂的吡哆醛磷酸连接酶，增加排泄的乙二醇大约 4 倍在 1 小时 （8 毫摩尔） 和 20 倍在 4 小时 （40 毫摩尔） 在 presence 的 0.2%CO2 在空气中。amount 的乙二醇排泄氨氧乙酸和 presence 的 0.2%CO2 在空气中等于或超过 amount 排泄在 0.2%CO2 在 O2 减去氨氧乙酸。CO2 和光被要求排泄乙二醇。氨氧乙酸也刺激光合作用乙二醇排泄在 atmosphere 的 0.2%CO2 在氮或氦气，虽然刺激不是很大，当空气或 O2 是 present.The 排泄乙二醇被转化为 H（2）和 CO2 由 combined 行动的 glycolic 氧化酶和甲酸 hydrogenlyase complex 发现大肠杆菌中总转化率为 80%。
