Department of Biology, York University, Downsview, Ontario M3J 1P3, Canada.
Plant Physiol. 1974 Mar;53(3):395-7. doi: 10.1104/pp.53.3.395.
Chlorella pyrenoidosa cells grown on 5% CO(2) excreted glycolate when incubated in light with 10 mm bicarbonate, but excreted no glycolate under the same conditions when they were maintained on air for 7 hours prior to the assay. Incubation of 5% CO(2)-grown and air-grown cells with 10 mm isonicotinyl hydrazide or 10 mm alpha-hydroxypyridinemethane sulfonate during the assay stimulated the excretion of glycolate by CO(2)-grown cells severalfold that of air-grown cells.Adaptation of CO(2)-grown Chlorella to growth on air did not affect the levels of glycolate dehydrogenase in the cells and did not affect the rate of dark oxidation and metabolism of exogeneous (14)C-glycolate by the cells. These results indicate that the lack of glycolate excretion by air-grown or air-adapted cells of Chlorella cannot be explained by a concomitant change in the level of glycolate dehydrogenase.
在光照下，用 10mM 碳酸氢盐培养在 5%CO2 上生长的栅藻细胞时会排出乙醇酸，但在检测前用空气维持 7 小时后，在相同条件下则不会排出乙醇酸。在检测过程中，用 10mM 异烟肼或 10mM α-羟基吡啶甲烷磺酸盐孵育 5%CO2 生长的和空气生长的细胞，会刺激 CO2 生长的细胞排出比空气生长的细胞多几倍的乙醇酸。适应空气生长的 CO2 培养的栅藻不会影响细胞中乙醇酸脱氢酶的水平，也不会影响细胞对黑暗氧化和外源（14C）乙醇酸代谢的速度。这些结果表明，空气生长或空气适应的栅藻细胞缺乏乙醇酸的排出不能用乙醇酸脱氢酶水平的同时变化来解释。
