脱落酸诱导培养植物细胞的抗冻性。

Abscisic Acid-induced freezing resistance in cultured plant cells.

机构信息

Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N OWO Canada.

出版信息

Plant Physiol. 1983 Sep;73(1):71-5. doi: 10.1104/pp.73.1.71.

DOI:10.1104/pp.73.1.71

PMID:16663189

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1066409/

Abstract

The effect of abscisic acid (ABA) on the cold hardiness of cell suspension was investigated. Cell suspension cultures of winter wheat (Triticum aestivum L. cv Norstar), winter rye (Secale cereale L. cv Cougar), and bromegrass Bromo inermis Leyss treated with 7.5 x 10(-5) molar ABA for 4 days at 20 degrees C could tolerate -30 degrees C, whereas the control cultures tolerated only -7 to -8 degrees C. The optimum concentration for increasing the cold hardiness of the cultures was 7.5 x 10(-5) molar. The degree of cold hardiness and the rate of hardening obtained by ABA treatment was significantly higher than that induced by low temperature alone. Of ten species tested, ABA was only effective on those cultures which were capable of cold hardening upon exposure to low temperatures. The results suggest that ABA bypasses the cold requirement for hardening and also suggests that ABA triggers the genetic system(s) responsible for inducing the hardening process.

摘要

研究了脱落酸（ABA）对细胞悬浮液抗寒性的影响。用 7.5×10（-5）摩尔 ABA 处理 4 天的冬小麦（Triticum aestivum L. cv Norstar）、冬黑麦（Secale cereale L. cv Cougar）和无芒雀麦（Bromo inermis Leyss）的细胞悬浮培养物在 20°C 下可耐受-30°C，而对照培养物仅能耐受-7 至-8°C。增加培养物抗寒性的最佳浓度为 7.5×10（-5）摩尔。ABA 处理提高抗寒性的程度和硬化速度明显高于单独低温诱导的程度。在测试的十种物种中，ABA 仅对那些在低温暴露下能够进行抗寒硬化的培养物有效。结果表明，ABA 绕过了硬化的低温要求，也表明 ABA 触发了负责诱导硬化过程的遗传系统。