College of Life Sciences, Jilin Agricultural University, Changchun, Jilin, China.
Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China.
GM Crops Food. 2022 Dec 31;13(1):131-141. doi: 10.1080/21645698.2022.2097831.
Maize ( L.) is a food crop sensitive to low temperatures. As one of the abiotic stress hazards, low temperatures seriously affect the yield of maize. However, the genetic basis of low-temperature adaptation in maize is still poorly understood. In this study, maize S-adenosylmethionine decarboxylase ) was localized to the nucleus. We used -mediated transformation technology to introduce the gene into an excellent maize inbred line variety GSH9901 and produced a cold-tolerant transgenic maize line. After three years of single-field experiments, the contents of polyamines (PAs), proline (Pro), malondialdehyde (MDA), antioxidant enzymes and ascorbate peroxidases (APXs) in the leaves of the transgenic maize plants overexpressing the gene significantly increased, and the expression of elevated and cold-responsive genes effectively increased. The agronomic traits of the maize overexpressing the gene changed, and the yield traits significantly improved. However, no significant changes were found in plant height, ear length, and shaft thickness. Therefore, enzymes can effectively improve the cold tolerance of maize.
玉米（L.）是一种对低温敏感的粮食作物。作为非生物胁迫危害之一，低温严重影响玉米的产量。然而，玉米低温适应的遗传基础仍知之甚少。本研究将玉米 S-腺苷甲硫氨酸脱羧酶定位于细胞核。我们使用介导的转化技术将基因导入优良的玉米自交系品种 GSH9901，并产生了耐低温的转基因玉米系。经过三年的单场实验，过表达基因的转基因玉米植株叶片中的多胺（PAs）、脯氨酸（Pro）、丙二醛（MDA）、抗氧化酶和抗坏血酸过氧化物酶（APXs）含量显著增加，和冷响应基因的表达有效增加。过表达基因的玉米的农艺性状发生了变化，产量性状显著提高。然而，株高、穗长和轴厚没有明显变化。因此，酶可以有效提高玉米的耐寒性。
