Laboratoire de Régulations Métaboliques et Différenciation des Plastes, Université Pierre et Marie Curie (Paris 6), 75230 Paris Cédex 05, France.
Plant Physiol. 1983 Sep;73(1):94-9. doi: 10.1104/pp.73.1.94.
A technique for the isolation and the purification of Capsicum annum L. var. Yolo Wonder chromoplasts is described. The degree of purity of the isolated chromoplasts is greatly improved by the absence of MgCl(2) in the extraction medium and in the gradient purification system, as shown by electron micrographs and the near absence of antimycin-insensitive NADH:cytochrome c reductase activity and succinate:cytochrome c reductase activity. Furthermore, phosphatidylserine was absent and the phosphatidylethanolamine content was reduced by a factor of 5 in these preparations, which were active in the synthesis of galactolipids, prenylquinones, and carotenoids.
描述了一种从辣椒（Capsicum annum L. var. Yolo Wonder）叶绿体中分离和纯化类囊体的方法。电镜照片显示，在提取介质和梯度纯化系统中不使用 MgCl(2)，极大地提高了分离类囊体的纯度，并且抗霉素 A 不敏感的 NADH：细胞色素 c 还原酶活性和琥珀酸：细胞色素 c 还原酶活性几乎不存在。此外，这些制剂中没有磷脂酰丝氨酸，磷脂酰乙醇胺的含量减少了 5 倍，它们在半乳糖脂、 prenylquinones 和类胡萝卜素的合成中具有活性。
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