Siddique Muhammad Asim, Grossmann Jonas, Gruissem Wilhelm, Baginsky Sacha
Institute of Plant Sciences, ETH Zürich, Universitätstrasse 2, CH-8092 Zürich, Switzerland.
Plant Cell Physiol. 2006 Dec;47(12):1663-73. doi: 10.1093/pcp/pcl033. Epub 2006 Nov 10.
We report a comprehensive proteome analysis of chromoplasts from bell pepper (Capsicum annuum L.). The combination of a novel strategy for database-independent detection of proteins from tandem mass spectrometry (MS/MS) data with standard database searches allowed us to identify 151 proteins with a high level of confidence. These include several well-known plastid proteins but also novel proteins that were not previously reported from other plastid proteome studies. The majority of the identified proteins are active in plastid carbohydrate and amino acid metabolism. Among the most abundant individual proteins are capsanthin/capsorubin synthase and fibrillin, which are involved in the synthesis and storage of carotenoids that accumulate to high levels in chromoplasts. The relative abundances of the identified chromoplast proteins differ remarkably compared with their abundances in other plastid types, suggesting a chromoplast-specific metabolic network. Our results provide an overview of the major metabolic pathways active in chromoplasts and extend existing knowledge about prevalent metabolic activities of different plastid types.
我们报告了对甜椒（辣椒属）叶绿体的全面蛋白质组分析。一种用于从串联质谱（MS/MS）数据中进行独立于数据库的蛋白质检测的新策略与标准数据库搜索相结合，使我们能够高度自信地鉴定出151种蛋白质。这些蛋白质包括几种著名的质体蛋白，也包括先前其他质体蛋白质组研究中未报道的新蛋白质。大多数鉴定出的蛋白质在质体碳水化合物和氨基酸代谢中具有活性。在最丰富的单个蛋白质中，有辣椒红素/辣椒玉红素合酶和原纤维蛋白，它们参与类胡萝卜素的合成和储存，类胡萝卜素在叶绿体中大量积累。与其他质体类型相比，鉴定出的叶绿体蛋白质的相对丰度有显著差异，这表明存在一个叶绿体特异性的代谢网络。我们的结果概述了叶绿体中活跃的主要代谢途径，并扩展了关于不同质体类型普遍代谢活动的现有知识。
