Department of Biochemistry, Mississippi State University, Mississippi, 39762.
Plant Physiol. 1984 Feb;74(2):455-6. doi: 10.1104/pp.74.2.455.
A radioimmunoassay was used to determine the globulin content of developing oat (Avena sativa L. var Coker 6622) seeds. The globulin content increased from 0.32 to 2.37 milligrams per seed from 2 to 21 days post anthesis. The amount of globulin did not increase from 21 to 30 days post anthesis. When the total protein of seeds harvested 21, 24, and 30 days post anthesis was measured by micro-Kjeldahl analysis, it was determined to be 3.15 milligrams per seed for each sampling date. When the amount of globulin per seed was compared to this value, the relative proportion of globulin to total seed protein was approximately 75%.
采用放射免疫法测定发育中的燕麦（Avena sativa L. var Coker 6622）种子的球蛋白含量。从授粉后 2 天到 21 天，球蛋白含量从每粒种子 0.32 毫克增加到 2.37 毫克。授粉后 21 天到 30 天，球蛋白含量没有增加。通过微量凯氏定氮法分析，测定授粉后 21、24 和 30 天收获的种子的总蛋白含量，每个采样日期均为每粒种子 3.15 毫克。将每粒种子的球蛋白含量与此值进行比较，球蛋白与总种子蛋白的相对比例约为 75%。
